Introgression of a Novel Ug99-Effective Stem Rust Resistance Gene into Wheat and Development of Dasypyrum villosum Chromosome-Specific Markers via Genotyping-by-Sequencing (GBS)
Introgression of a Novel Ug99-Effective Stem Rust Resistance Gene into Wheat and Development of Dasypyrum villosum Chromosome-Specific Markers via Genotyping-by-Sequencing (GBS)
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DOI：
10.1094/pdis-05-18-0831-re
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发表时间：
2019-06-01
期刊：
影响因子：
4.5
通讯作者：
Pumphrey, Michael O.
中科院分区：
文献类型：
--
作者：
Ando, Kaori;Krishnan, Vandhana;Pumphrey, Michael O.
Dasypyrum villosum 是普通小麦 (Triticum aestivum L.) 的野生近缘种，对小麦柄锈菌 (Puccinia graminis f) 具有抗性。小麦，茎锈病的致病因子，包括高毒力品种 TTKSK (Ug99)。为了转移阻力，T. duru-D。绒毛双倍体最初被开发并用作创建小麦-D 的桥梁。绒毛渗入线。使用保守直系同源集 (COS) 标记来鉴定 D. villosum 染色体渗入系，然后将其置于幼苗 P. graminis f 中。使用 TTKSK 品种筛选小麦抗性。通过组合基因组原位和荧光原位分析进一步表征了经过 COS 标记验证的携带 2V 染色体且具有 TTKSK 抗性的品系，以确认单体替换品系 MS2V(2D) (20 '' + 1' 2V[2D])。这是第一份在2V上抗锈病的报道，暂时命名为SrTA10276-2V。为了促进该基因在小麦改良中的应用，对一整套先前开发的小麦绒毛二体附加系进行了测序分析基因分型，以开发绒毛染色体特异性标记。平均每条染色体鉴定出 350 个标记。这些标记可用于开发对小麦改良感兴趣的 D. villusum 衍生基因的诊断标记。
Dasypyrum villosum is a wild relative of common wheat (Triticum aestivum L.) with resistance to Puccinia graminis f. tritici, the causal agent of stem rust, including the highly virulent race TTKSK (Ug99). In order to transfer resistance, T. duru-D. villosum amphiploids were initially developed and used as a bridge to create wheat-D. villosum introgression lines. Conserved ortholog set (COS) markers were used to identify D. villosum chromosome introgression lines, which were then subjected to seedling P. graminis f. tritici resistance screening with race TTKSK. A COS marker-verified line carrying chromosome 2V with TTKSK resistance was further characterized by combined genomic in situ and fluorescent in situ analyses to confirm a monosomic substitution line MS2V(2D) (20 '' + 1' 2V[2D]). This is the first report of stem rust resistance on 2V, which was temporarily designated as SrTA10276-2V. To facilitate the use of this gene in wheat improvement, a complete set of previously developed wheat D. villosum disomic addition lines was subjected to genotyping-by-sequencing analysis to develop D. villosum chromosome-specific markers. On average, 350 markers per chromosome were identified. These markers can be used to develop diagnostic markers for D. villosum-derived genes of interest in wheat improvement.