Dietary (n-3) long chain polyunsaturated fatty acids prevent sucrose-induced insulin resistance in rats
Dietary (n-3) long chain polyunsaturated fatty acids prevent sucrose-induced insulin resistance in rats
复制标题
DOI：
10.1093/jn/135.11.2634
复制
发表时间：
2005-11-01
影响因子：
4.2
通讯作者：
Acharya, V
中科院分区：
文献类型：
--
作者：
Ghafoorunissa;Ibrahim, A;Acharya, V
本研究旨在确定用（n-3）长链多不饱和脂肪酸（LCPUFA）替代亚油酸，从而降低（n-6）：（n-3）脂肪酸比例对蔗糖诱导的大鼠胰岛素抵抗的影响。断奶雄性Wistar大鼠饲喂含酪蛋白的饲料100 g/kg脂肪12 wk。用蔗糖（SU）代替淀粉（ST）诱导胰岛素抵抗。用花生油、棕榈油蛋白和鱼油配制膳食脂肪，以提供以下比例的（n-6）：（n-3）脂肪酸：210（ST-210、SU-210）、50（SU-50）、10（SU-10）和5（SU-5）。与淀粉（ST-210）相比，蔗糖喂养（SU-210）显着增加血浆胰岛素和甘油三酯浓度和血浆胰岛素曲线下面积（AUC）响应于口服葡萄糖负荷。与饲喂ST-210的大鼠相比，饲喂SU-210的大鼠分离的脂肪细胞具有更高的脂解速率、更低的胰岛素刺激的葡萄糖转运和更低的胰岛素介导的抗脂解作用。降低蔗糖喂养大鼠（SU-10和SU-5）的饮食（n-6）：（n-3）比例使血糖负荷后的血浆胰岛素浓度和胰岛素AUC正常化。在饲喂SU-50、SU-10和SU-5的大鼠中，蔗糖诱导的血浆甘油三酯浓度升高恢复正常。此外，在喂食SU-5和SU-10的大鼠中，蔗糖诱导的脂肪细胞脂解和抗脂解的改变被部分逆转，葡萄糖转运得到改善。在隔膜磷脂中，降低饮食中的（n-6）：（n-3）比例增加了（n-3）LCPUFA的浓度，同时降低了（n-6）LCPUFA的浓度。这些结果表明，2.6 g/kg饲料水平的（n-3）LCPUFA [0.56%能量（n-3）LCPUFA，（n-6）：（n-3）比率= 10]可通过改善外周胰岛素敏感性来预防蔗糖诱导的胰岛素抵抗。
This study was designed to determine the effect of substituting (n-3) long-chain PUFAs (LCPUFAs) for linoleic acid and hence decreasing the (n-6):(n-3) fatty acid ratio on sucrose-induced insulin resistance in rats. Weanling male Wistar rats were fed casein-based diets containing 100 g/kg fat for 12 wk. Insulin resistance was induced by replacing starch (ST) with sucrose (SU). The dietary fats were formulated with groundnut oil, palmolein, and fish oil to provide the following ratios of (n-6):(n-3) fatty acids: 210 (ST-210, SU-210), 50 (SU-50), 10 (SU-10), and 5 (SU-5). Compared with starch (ST-210), sucrose feeding (SU-210) significantly increased the plasma insulin and triglyceride concentrations and the plasma insulin area under the curve (AUC) in response to an oral glucose load. Adipocytes isolated from rats fed SU-210 had greater lipolytic rate, lower insulin stimulated glucose transport, and lower insulin-mediated antilipolysis than those from rats fed ST-210. Decreasing the dietary (n-6):(n-3) ratio in sucrose-fed rats (SU-10 and SU-5) normalized the plasma insulin concentration and the AUC of insulin after a glucose load. The sucrose-induced increase in plasma triglyceride concentration was normalized in rats fed SU-50, SU-10 and SU-5. Further, sucrose-induced alterations in adipocyte lipolysis and antilipolysis were partially reversed and glucose transport improved in rats fed diets SU-5 and SU-10. In diaphragm phospholipids, decreasing the (n-6):(n-3) ratio in the diet increased the concentration of (n-3) LCPUFAs with concomitant decreases in the concentration of (n-6) LCPUFAs. These results suggest that (n-3) LCPUFAs at a level of 2.6 g/kg diet [0.56% energy (n-3) LCPUFAs, (n-6):(n-3) ratio = 10] may prevent sucrose-induced insulin resistance by improving peripheral insulin sensitivity.