Culture Under Low Physiological Oxygen Conditions Improves the Stemness and Quality of Induced Pluripotent Stem Cells
Culture Under Low Physiological Oxygen Conditions Improves the Stemness and Quality of Induced Pluripotent Stem Cells
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DOI：
10.1002/jcp.24389
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发表时间：
2013-11-01
影响因子：
5.6
通讯作者：
Li, Tao-Sheng
中科院分区：
文献类型：
--
作者：
Guo, Chao-Wan;Kawakatsu, Miho;Li, Tao-Sheng
(2))已经证明，在合适的条件下培养能够改善细胞的干性和基因组稳定性。我们研究了低氧培养是否有利于诱导多能干细胞（iPS）的培养。(2)、5%O-2或20%O-2，并平行维持2个月。(2)比20%的氧气浓度要高在各种氧气条件下扩增的所有这些iPS集落对Oct 3/4、Nanog、SSEA-4和ALP呈阳性染色。然而，Western blot分析表明，在2.5%和5%O-2培养的iPS细胞表达的Nanog显著高于20%O-2培养的细胞，但53 BP 1显著低于20%O-2培养的细胞。阵列CGH的数据显示没有显着的染色体异常，虽然一些基因参与细胞和代谢过程中的低氧培养扩增，特别是在2.5%O-2。我们的数据表明，低生理氧培养可以提高干细胞和iPS细胞的质量，结果可能与参与代谢和细胞过程的基因扩增有关。长期培养将是必要的，以确认是否低生理氧水平也提高基因组的稳定性。J.细胞。228：2159-2166，2013。(c)2013 Wiley Periodicals，Inc.
(2)) conditions has been demonstrated to improve the stemness and genomic stability of the cells. We investigated whether low-oxygen culture would be beneficial for the culture of induced pluripotent stem (iPS) cells. (2), 5% O-2, or 20% O-2 and maintained for 2 months in parallel. (2) than at 20% O-2. All of these iPS colonies that expanded under the various oxygen conditions stained positively for Oct3/4, Nanog, SSEA-4, and ALP. However, Western blot analysis showed that the iPS cells cultured at 2.5% and 5% O-2 expressed significantly more Nanog but less 53BP1 than those cultured at 20% O-2. Data from an array CGH showed no significant chromosomal abnormalities, although some genes involved in cellular and metabolic processes were amplified in the low oxygen culture, particularly at 2.5% O-2. Our data suggest that low physiological oxygen culture could improve the stemness and quality of iPS cells, a result that might be associated with the amplification of genes involved in metabolic and cellular processes. Long-term culture will be necessary to confirm whether low physiological oxygen levels also improve genomic stability. J. Cell. Physiol. 228: 2159-2166, 2013. (c) 2013 Wiley Periodicals, Inc.