The effects of pretreatment with glycyrrhizin and glycyrrhetinic acid on the retrorsine-induced hepatotoxicity in rats
The effects of pretreatment with glycyrrhizin and glycyrrhetinic acid on the retrorsine-induced hepatotoxicity in rats
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DOI：
10.1016/s0041-0101(98)00263-3
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发表时间：
1999-09-01
期刊：
影响因子：
2.8
通讯作者：
Cheng, TY
中科院分区：
文献类型：
--
作者：
Lin, G;Nnane, IP;Cheng, TY
多种中草药含有肝脏毒性的吡咯里西丁生物碱(PAS)，常对人体造成急性和慢性肝脏损害。甘草是一种已知的抗肝炎药物，通常与含PA的草药同时使用，但这种联合使用所引起的肝毒性并不明显。研究甘草主要生物活性成分甘草酸(GL)和18-β-甘草次酸(CA)对苯丙氨酸(PA)所致大鼠肝毒性的影响。用一种典型的强肝毒性PA--逆转录肾上腺素(RET)对大鼠造成肝损伤。在逆转录病毒激发前单次给予GL或GA并未显示出肝脏保护作用。然而，当大鼠预先给予甘草酸甘油(200 mg/kg/d，i.p.)或GA(10 mg/kg/d，i.p.)连续三天的逆皮质醇暴露前，血清谷草转氨酶和谷丙转氨酶水平显著降低。血清转氨酶水平基本恢复正常(GOT：56+/-2(对照组)、104+/-5(RET)、+/-3(GL+RET)和59+/-3(GA+RET))。GPT：40+/-2(对照组)、90+/-7(RET)、45+/-2(GL+RET)和45+/-4(GA+RET)SF单位/ml)。此外，未观察到广泛的肝细胞损害。结果表明，GL或GA预处理3d对反式维甲酸诱导的大鼠肝损伤有保护作用。(C)1999爱思唯尔科学有限公司，保留所有权利。
A wide variety of medicinal herbs contain hepatotoxic pyrrolizidine alkaloids (PAs), and often cause acute and chronic liver damages in man. Liquorice, a known antihepatitis, is commonly used with PA-containing herbs concurrently, and hepatotoxicity induced by such combined uses was not pronounced. The present study is to investigate effects of glycyrrhizin (GL) and 18 beta-glycyrrhetinic acid (CA), the major biologically active ingredients of liquorice, against PA-induced hepatotoxicity in rats.Single dose (35 mg/kg, i.p.) of retrorsine (RET), a typical potent hepatotoxic PA, was given to rats to induce liver injury. A single dose pretreatment with GL or GA prior to retrorsine challenge did not show hepatoprotection. However, when rats were pretreated with either GL (200 mg/kg/day, i.p.) or GA (10 mg/kg/day, i.p.) for three consecutive days prior to retrorsine exposure, the elevated serum GOT and GPT levels induced by retrorsine were significantly reduced. Serum levels of transaminases almost returned to normal (GOT: 56 +/- 2 (control), 104 +/- 5 (RET), 64 +/- 3 (GL + RET) and 59 +/- 3 (GA + RET). GPT: 40 +/- 2 (control), 90 +/- 7 (RET), 45 +/- 2 (GL + RET) and 45 +/- 4 (GA + RET) SF units/ml). Furthermore, no extensive hepatocellular damages were observed. The results demonstrated that a three-day pretreatment with either GL or GA exhibited protective effect on retrorsine-induced liver damage in rats. (C) 1999 Elsevier Science Ltd,All rights reserved.