Characterization of glucansucrase and dextran from Weissella sp TN610 with potential as safe food additives
Characterization of glucansucrase and dextran from Weissella sp TN610 with potential as safe food additives
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DOI：
10.1016/j.ijbiomac.2012.09.014
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发表时间：
2013-01-01
影响因子：
8.2
通讯作者：
Chouayekh, Hichem
中科院分区：
文献类型：
--
作者：
Bejar, Wacim;Gabriel, Valerie;Chouayekh, Hichem
来源于梨的Weissella sp. TN 610产生胞外糖基转移酶活性，负责从蔗糖合成可溶性胞外多糖。酸和葡聚糖酶催化水解表明，合成的聚合物是葡聚糖。根据H-1和C-13 NMR分析，由TN 610产生的葡聚糖是由96%的α-（1 -> 6）和4%的α-（1 -> 3）键组成的线性葡聚糖。酶谱分析证实在来自TN 610的无细胞上清液中存在约180 kDa的独特葡聚糖蔗糖酶。该粗酶在37 ℃和pH 5下具有最佳活性，具有作为食品添加剂的应用潜力，因为它催化补充蔗糖的牛奶中的葡聚糖合成，使其固化。通过PCR从TN 610中扩增出一个4257 bp的产物，该产物对应于成熟的葡聚糖蔗糖酶基因。它编码的1418个残基的多肽具有计算的分子量为156.089 kDa，并表现出96%和95%的同一性，从发酵乳杆菌Kg 3和Weissella cibaria CMU的葡聚糖蔗糖酶，分别。(C)2012爱思唯尔有限公司版权所有。
Pear-derived Weissella sp. TN610 produced extracellular glycosyltransferase activity responsible for the synthesis of soluble exopolysaccharide from sucrose. Acid and dextranase-catalyzed hydrolysis revealed that the synthesized polymer was a glucan. According to H-1 and C-13 NMR analysis, the glucan produced by TN610 was a linear dextran made of 96% alpha-(1 -> 6) and 4% alpha-(1 -> 3) linkages. Zymogram analysis confirmed the presence of a unique glucansucrase of approximately 180 kDa in the cell-free supernatant from TN610. The crude enzyme, optimally active at 37 degrees C and pH 5, has promising potential for application as a food additive since it catalyzes dextran synthesis in sucrose-supplemented milk, allowing its solidification. A 4257-bp product corresponding to the mature glucansucrase gene was amplified by PCR from TN610. It encoded a polypeptide of 1418 residues having a calculated molecular mass of 156.089 kDa and exhibiting 96% and 95% identity with glucansucrases from Lactobacillus fermentum Kg3 and Weissella cibaria CMU, respectively. (C) 2012 Elsevier B.V. All rights reserved.