Exploring the structural diversity of mammalian carbohydrates ("Glycospace") by statistical databank analysis
Exploring the structural diversity of mammalian carbohydrates ("Glycospace") by statistical databank analysis
复制标题
DOI：
10.1021/cb700178s
复制
发表时间：
2007-10-01
影响因子：
4
通讯作者：
Seeberger, Peter H.
中科院分区：
文献类型：
--
作者：
Werz, Daniel B.;Ranzinger, Rene;Seeberger, Peter H.
对存放在数据库 GLYCOSSCIENCES.de 中的三大类哺乳动物碳水化合物（主要是糖肽以及 O-和 N-连接聚糖）的多样性进行了统计分析。对大小、链长和分支复杂性进行了评估，结果表明寡糖平均由大约八个单糖单元组成。大约四分之一的寡糖是严格线性的，其余的至少有一次支化。葡萄糖胺、半乳糖和甘露糖占主导地位，占哺乳动物寡糖框架内单糖的 75%。 α-连接唾液酸、α-连接岩藻糖和P-连接半乳糖装饰大部分还原末端。葡萄糖作为哺乳动物中最丰富的碳水化合物，在这些结构中仅发挥非常小的作用。特别强调分析单糖单元在寡聚框架内的连接方式。仅 11 个单糖连接就占所有连接的 75% 以上。因此，自然界中发现的结构组合的数量，即占据哺乳动物糖空间的部分，比预期的要少得多。因此，提出了一组潜在的寡糖组装构建模块。这种潜在的构建模块集与 GLYCOSSCIENCES.de 数据库中可访问的 3299 个哺乳动物碳水化合物结构相关。构建 3299 种哺乳动物寡糖中的 75% 仅需要 36 个结构单元。
The diversity of three major classes of mammalian carbohydrates, mainly glycotipids and O- and N-linked glycans, deposited in the databank GLYCOSCIENCES.de was subjected to statistical analyses. Size, chain length, and branching complexity were accessed and revealed that the average oligosaccharide is composed of about eight monosaccharide units. About a quarter of all oligosaccharides are strictly linear, and the remainder are branched at least once. Glucosamine, galactose, and mannose are dominating and comprise 75% of the monosaccharides within mammalian oligosaccharicle frameworks. alpha-linked sialic acid, alpha-linked fucose, and P-linked galactose decorate the majority of reducing termini. Glucose as the most abundant carbohydrate in mammals plays only a very minor role within these structures. Particular emphasis was placed on analyzing the way the monosaccharide units are linked within the oligomeric framework. just 11 monosaccharide connections account for >75% of all linkages. Thus, the number of structural combinations found in nature, the part of the occupied mammalian glycospace, is much smaller than expected. As a result, a potential set of building blocks for oligosaccharicle assembly is presented. This potential building block set was correlated with the accessible 3299 mammalian carbohydrate structures in the GLYCOSCIENCES.de databank. Only 36 building blocks are required to construct 75% of the 3299 mammalian oligosaccharides.