Injectable and bioresorbable calcium phosphate delivery system with gentamicin sulphate for treatment of bone diseases: in vitro study
Injectable and bioresorbable calcium phosphate delivery system with gentamicin sulphate for treatment of bone diseases: in vitro study
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DOI：
10.1179/1743676111y.0000000053
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发表时间：
2011-11-01
影响因子：
2.2
通讯作者：
Khoshroo, K.
中科院分区：
文献类型：
--
作者：
Haghbin-Nazarpak, M.;Moztarzadeh, F.;Khoshroo, K.
在本研究中，磷酸钙骨水泥（CPC）糊剂通过将由4 wt-%的正磷酸氢二钠组成的骨水泥液体与由β-磷酸三钙和磷酸二氢钙一水合物组成的骨水泥粉末组合来制备。在体外研究中，通过高效液相色谱法测定了硫酸庆大霉素（GS）从磷酸盐缓冲盐水溶液中浸泡的CPC标准圆柱形样品中在一段时间内的释放。此外，作为生物医学特性的代表，测量凝固时间、可注射性和抗压强度，以检测CPC与GS混合时的变化。此外，含样品的磷酸盐缓冲盐水溶液的pH值逐渐下降，直至达到平衡pH。总体而言，体外释放研究的结果显示，其在第15天达到最大水平，为GS初始值的35%，表明骨水泥糊剂与抗生素之间没有发生不可逆结合。
In this present research, calcium phosphate cement (CPC) paste was prepared by combining cement liquids comprised of 4 wt-% disodium hydrogen orthophosphate with cement powders that consisted of beta-tricalcium phosphate and monocalcium phosphate monohydrate. In the in vitro study, the release of gentamicin sulphate (GS) from standard cylindrical samples of the prepared CPC immersion in phosphate buffered saline solution was measured by high performance liquid chromatography test over a period of time. In addition, as a representative for biomedical properties, setting time, injectability and compressive strength were measured in order to detect changes when mixing CPC with GS. In addition, the pH values of the phosphate buffered saline solution containing samples descended gradually until reaching equilibrium pH. Overall, the results from in vitro release study showed that it reached its maximum level, which was 35% of the initial value of the GS on day 15, suggesting no irreversible binding occurred between the cement paste and the antibiotic.