Detection of maternal-fetal microchimerism in the inflammatory lesions of patients with Sjögren's syndrome
Detection of maternal-fetal microchimerism in the inflammatory lesions of patients with Sjögren's syndrome
复制标题
干燥综合征患者炎症病变中母胎微嵌合体的检测
DOI：
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发表时间：
2002
影响因子：
27.4
通讯作者：
Hirohito Tsubouchi
中科院分区：
文献类型：
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作者：
M. Kuroki;A. Okayama;Seiji Nakamura;Takashi Sasaki;K. Murai;R. Shiba;M. Shinohara;Hirohito Tsubouchi
背景资料：母胎微嵌合体与自身免疫性疾病之间可能存在的关系与慢性移植物抗宿主病（cGVHD）有一些相似之处。目的：研究女性干燥综合征（SS）患者体内是否存在男性DNA细胞，因为SS与cGVHD具有相似的临床特征。研究方法：从56例女性SS患者的27份外周血单个核细胞（PBMC）、42份唇唾液腺（LSG）活检标本和9份支气管肺泡灌洗液（BALF）细胞中提取DNA。通过巢式聚合酶链反应（PCR）和荧光原位杂交（FISH）确定男性DNA的存在。结果：56例女性SS患者中，42例至少有一个男孩。在这42名患者中，22个PBMC中没有一个，但10/28（36%）LSG样品通过PCR检测Y染色体特异性序列为阳性（p=0.0013）。10例非SS患者的LSG标本中未检测到Y染色体特异性序列。在BALF样本中，2/9（22%）的SS患者通过PCR检测呈阳性。FISH结果显示，3例SS女性患者的LSG标本中均存在Y染色体细胞。结论：本研究首次发现母胎微嵌合体存在于SS女性患者的唾液腺和肺中。非宿主细胞的存在下，在炎症病变，但不是在外周血表明母胎微嵌合体在SS的发病机制中可能发挥作用。
Background: A possible relation between maternal-fetal microchimerism and autoimmune diseases with some similarities to chronic graft versus host disease (cGVHD) has been reported. Objective: To investigate whether cells with male DNA exist in female patients with Sjögren's syndrome (SS) as SS has clinical features similar to those of cGVHD. Methods: DNA was extracted from 27 samples of peripheral blood mononuclear cells (PBMC), 42 biopsy samples of labial salivary glands (LSG), and nine samples of bronchoalveolar lavage fluid (BALF) cells from 56 female patients with SS. The presence of male DNA was determined by nested polymerase chain reaction (PCR) and by fluorescence in situ hybridisation (FISH). Results: Among 56 female patients with SS, 42 patients had at least one male child. Among those 42 patients, none of the 22 PBMC but 10/28 (36%) LSG samples tested positive by PCR for the Y chromosome-specific sequence (p=0.0013). The Y chromosome-specific sequence was not detected in the samples of LSG in 10 patients without SS. In the BALF samples 2/9 (22%) patients with SS tested positive by PCR. Cells containing the Y chromosome were shown to exist in all the LSG specimens from three female patients with SS by FISH. Conclusions: Maternal-fetal microchimerism was shown for the first time to exist in the salivary glands and lungs of female patients with SS in this study. The presence of non-host cells in the inflammatory lesions but not in the peripheral blood suggests a possible role for maternal-fetal microchimerism in the pathogenesis of SS.
免疫活性输血受者中供体白细胞亚群的存活：严重创伤患者中频繁出现长期微嵌合现象。
影响因子：
20.3
作者：
Lee,TH;Paglieroni,T;Ohto,H;Holland,PV;Busch,MP
通讯作者：
Busch,MP