Central peptidergic ensembles associated with organization of an innate behavior
Central peptidergic ensembles associated with organization of an innate behavior
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DOI：
10.1073/pnas.0603459103
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发表时间：
2006-09-19
影响因子：
11.1
通讯作者：
Adams, Michael E.
中科院分区：
文献类型：
--
作者：
Kim, Young-Joon;Zitnan, Dusan;Adams, Michael E.
在每个发育阶段的末期，昆虫执行蜕皮序列，这是一种脱落旧角质层所必需的先天行为。蜕皮触发激素(ETH)通过对中枢神经系统的直接作用启动这些行为。在这里，我们发现了羽毛虫的ETH受体(ETHR)基因，该基因编码两种亚型的GPCR(eTHR-A和eTHR-B)。ETHs在中枢神经系统的表达与中枢神经系统对ETHs的敏感性和行为能力的获得完全一致。ETHR-A存在于不同的神经元网络中，产生兴奋性和抑制性神经肽，这些神经肽似乎是行为调节的下游信号。这些多肽包括：血管紧张素、甲壳类心脏活性多肽、降钙素样利尿激素、CRF样利尿激素41和30、羽化激素、激肽、肌抑制肽、神经肽F和短神经肽F，特别是腹神经节细胞L-3、L-4共表达激肽、激肽41和短神经肽30，共同诱导假想的蜕变前节律。腹神经节的IN704神经元共表达CCAP和MIPs，它们的联合作用启动了蜕皮运动程序。ETHR-A也在脑腹内侧细胞表达，其释放的EH增加了CCAP/MIP神经元的兴奋性。这些发现提供了关于如何通过招募肽共递质神经元来协调先天的、中央模式的行为的见解。
At the end of each developmental stage, insects perform the ecdysis sequence, an innate behavior necessary for shedding the old cuticle. Ecdysis triggering hormones (ETHs) initiate these behaviors through direct actions on the CNS. Here, we identify the ETH receptor (ETHR) gene in the moth Manduca sexta, which encodes two subtypes of GPCR (ETHR-A and ETHR-B). Expression of ETHRs in the CNS coincides precisely with acquisition of CNS sensitivity to ETHs and behavioral competence. ETHR-A occurs in diverse networks of neurons, producing both excitatory and inhibitory neuropeptides, which appear to be downstream signals for behavior regulation. These peptides include allatostatins, crustacean cardioactive peptide (CCAP), calcitonin-like diuretic hormone, CRF-like diuretic hormones (DHs) 41 and 30, eclosion hormone, kinins, myoinhibitory peptides (MIPs), neuropeptide F, and short neuropeptide F. In particular, cells L-3,L-4 in abdominal ganglia coexpress kinins, DH41, and DH30, which together elicit the fictive preecdysis rhythm. Neurons IN704 in abdominal ganglia coexpress CCAP and MIPs, whose joint actions initiate the ecdysis motor program. ETHR-A also is expressed in brain ventromedial cells, whose release of EH increases excitability in CCAP/MIP neurons. These findings provide insights into how innate, centrally patterned behaviors can be orchestrated via recruitment of peptide cotransmitter neurons.