Continuously manufactured magnetic polymersomes - a versatile tool (not only) for targeted cancer therapy
Continuously manufactured magnetic polymersomes - a versatile tool (not only) for targeted cancer therapy
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DOI：
10.1039/c3nr02190d
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发表时间：
2013-01-01
期刊：
影响因子：
6.7
通讯作者：
Maskos, Michael
中科院分区：
文献类型：
--
作者：
Bleul, Regina;Thiermann, Raphael;Maskos, Michael
微混合器技术用于制备双重装载抗癌药物喜树碱和磁性纳米颗粒的聚合物囊泡（Pluronic（R）L-121）。透射电子显微镜证实磁性纳米颗粒的成功掺入。动态光散射测量结果表明，一个相对较窄的混合聚合物囊泡的大小分布。喜树碱聚合物囊泡降低前列腺癌细胞（PC-3）72小时后测量的细胞活力显着，而无药物聚合物囊泡显示没有细胞毒性作用。进行癌症靶向肽（铃蟾肽）以及荧光标记（Alexa Fluor（R）647）与杂交聚合物囊泡的共价连接，并通过流式细胞术和共聚焦显微镜显示特异性细胞结合和内化。弛豫测量清楚地证明了磁性聚合物囊泡产生显著的T2加权MRI对比度的能力，并可能允许直接监测聚合物囊泡的生物分布。微混合器技术作为一种简单、快速、有效的方法来制造作为治疗诊断药物递送装置的杂化聚合物囊泡，是从基础研究到个性化医疗的又一步。
Micromixer technology was used to prepare polymeric vesicles (Pluronic (R) L-121) dual loaded with the anticancer drug camptothecin and magnetic nanoparticles. Successful incorporation of the magnetic nanoparticles was confirmed by transmission electron microscopy. Dynamic light scattering measurements showed a relatively narrow size distribution of the hybrid polymersomes. Camptothecin polymersomes reduced the cell viability of prostate cancer cells (PC-3) measured after 72 h significantly, while drug-free polymersomes showed no cytotoxic effects. Covalent attachment of a cancer targeting peptide (bombesin) as well as a fluorescent label (Alexa Fluor (R) 647) to the hybrid polymersomes was performed and specific cell binding and internalization were shown by flow cytometry and confocal microscopy. Relaxometry measurements clearly demonstrated the capacity of magnetic polymersomes to generate significant T2-weighted MRI contrast and potentially allow for direct monitoring of the biodistribution of the polymersomes. Micromixer technology as an easy, fast and efficient way to manufacture hybrid polymersomes as theranostic drug delivery devices is a further step from basic research to personalized medicine.