Next-generation whole genome sequencing of dengue virus.
Next-generation whole genome sequencing of dengue virus.
复制标题
登革热病毒的下一代全基因组测序。
DOI：
10.1007/978-1-4939-0348-1_12
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发表时间：
2014
影响因子：
--
通讯作者：
M. Hibberd
中科院分区：
文献类型：
--
作者：
P. Aw;P. F. de Sessions;A. Wilm;L. Hoang;N. Nagarajan;O. Sessions;M. Hibberd
RNA病毒因其能够快速适应宿主免疫系统和/或抗病毒药物的选择性压力而臭名昭著。这种适应性可能是由于其依赖于 RNA 的 RNA 聚合酶的易于出错的特性 [1, 2]。登革热病毒是正链 RNA 病毒黄病毒科的一员，也具有这些容易出错的特征 [3]。利用高通量、大规模并行测序方法或下一代测序 (NGS)，我们现在可以准确量化这些病毒群体，并跟踪这些群体在单次感染过程中的变化。本章的目的有两个：描述测序前样品制备所需的方法以及描述所得数据所需的生物信息学分析。
RNA viruses are notorious for their ability to quickly adapt to selective pressure from the host immune system and/or antivirals. This adaptability is likely due to the error-prone characteristics of their RNA-dependent, RNA polymerase [1, 2]. Dengue virus, a member of the Flaviviridae family of positive-strand RNA viruses, is also known to share these error-prone characteristics [3]. Utilizing high-throughput, massively parallel sequencing methodologies, or next-generation sequencing (NGS), we can now accurately quantify these populations of viruses and track the changes to these populations over the course of a single infection. The aim of this chapter is twofold: to describe the methodologies required for sample preparation prior to sequencing and to describe the bioinformatics analyses required for the resulting data.
影响因子：
6.7
作者：
Henn MR;Boutwell CL;Charlebois P;Lennon NJ;Power KA;Macalalad AR;Berlin AM;Malboeuf CM;Ryan EM;Gnerre S;Zody MC;Erlich RL;Green LM;Berical A;Wang Y;Casali M;Streeck H;Bloom AK;Dudek T;Tully D;Newman R;Axten KL;Gladden AD;Battis L;Kemper M;Zeng Q;Shea TP;Gujja S;Zedlack C;Gasser O;Brander C;Hess C;Günthard HF;Brumme ZL;Brumme CJ;Bazner S;Rychert J;Tinsley JP;Mayer KH;Rosenberg E;Pereyra F;Levin JZ;Young SK;Jessen H;Altfeld M;Birren BW;Walker BD;Allen TM
通讯作者：
Allen TM