Effects of dietary menhaden oil and retinyl acetate on the growth of DU 145 human prostatic adenocarcinoma cells transplanted into athymic nude mice.
Effects of dietary menhaden oil and retinyl acetate on the growth of DU 145 human prostatic adenocarcinoma cells transplanted into athymic nude mice.
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膳食鲱鱼油和乙酸视黄酯对移植到无胸腺裸鼠的 DU 145 人前列腺腺癌细胞生长的影响。
DOI：
10.1093/carcin/9.4.603
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发表时间：
1988
期刊：
影响因子：
4.7
通讯作者：
Cohen,LA
中科院分区：
文献类型：
--
作者：
Rose,DP;Cohen,LA
在无胸腺裸鼠中研究了饲喂富含ω-3脂肪酸的鲱鱼油（MO）或补充维生素A（如醋酸视黄酯（RA））对DU 145人前列腺癌细胞生长的影响。给小鼠喂食含有23%玉米油（CO）、17%MO和6%CO的混合物或23%CO加RA的AIN-76 A饮食。辐照灭菌后，发现补充RA的饮食中含有的维生素A的量是对照饮食中的1.15倍。每个饮食组中有24只小鼠。开始饲喂实验饲料3周后，将1× 106或5× 106 DU 145细胞接种到12只动物的亚组中，并在6周内观察实体瘤的出现和生长。无论接种量如何，饲喂MO加CO的小鼠与仅饲喂CO的小鼠之间的肿瘤潜伏期没有显著差异。然而，接种5× 106个细胞并喂食补充RA的CO饮食的小鼠（17天后91%）与接受相同肿瘤细胞负荷但喂食未补充CO饮食的小鼠（17天后55%）相比，可触及肿瘤的出现更快。与CO对照组相比，接种1× 106个细胞并喂食含MO饮食的小鼠实体瘤生长显著减慢;在接种5 × 106个细胞的动物中，这种效应不明显。RA的补充导致肿瘤生长加速，同样，仅在接种1 × 106细胞的组中达到统计学显著性。
The effects of feeding menhaden oil (MO), rich in ω-3 fatty acids, or supplemental vitamin A [as retinyl acetate (RA)], on the growth of DU 145 human prostate cancer cells were studied in athymic nude mice. The mice were fed AIN-76A diets containing either 23% corn oil (CO), a mixture of 17% MO and 6% CO, or 23% CO plus RA. After irradiation sterilization, the RA-supplemented diet was found to contain ∼15 times the amount of vitamin A present in the control diet. There were 24 mice in each dietary group. Three weeks after commencement of feeding the experimental diets, 1×106or 5×106DU 145 cells were inoculated into subgroups of 12 animals, and the appearance and growth of solid tumors followed over a 6-week period. There was no significant difference in tumor latency between mice fed MO plus CO, and those fed CO alone, regardless of the inoculum size. However, the appearance of palpable tumors was more rapid in mice inoculated with 5×106cells and fed the RA-supplemented CO diet (91% after 17 days) compared with mice receiving the same tumor cell load but fed the unsup-plemented CO diet (55% after 17 days). Growth of the solid tumors was retarded significantly in mice inoculated with 1×106cells and fed the MO-containing diet compared with the CO controls; this effect was not evident in animals who received 5 ∼ 106cells. RA supplementation caused accelerated tumor growth, which, again, only achieved statistical significance in thegroup inoculated with 1 ∼ 106cells.