Genetic Diversity and Structure of Lolium Species Surveyed on Nuclear Simple Sequence Repeat and Cytoplasmic Markers.
Genetic Diversity and Structure of Lolium Species Surveyed on Nuclear Simple Sequence Repeat and Cytoplasmic Markers.
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通过核简单序列重复和细胞质标记调查黑麦草物种的遗传多样性和结构
DOI：
10.3389/fpls.2017.00584
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发表时间：
2017
影响因子：
5.6
通讯作者：
Cai H
中科院分区：
文献类型：
--
作者：
Guan X;Yuyama N;Stewart A;Ding C;Xu N;Kiyoshi T;Cai H
利用32个核单核苷酸重复序列(SSR)标记和7个细胞质基因标记，对9个黑麦草属162份材料的357个个体进行了遗传多样性和群体结构分析。核型SSR标记和细胞质基因标记平均每个座位的等位基因数分别为23.59和5.29，平均PIC值分别为0.83和0.54。分子方差分析表明，在32个核SSR标记和6个叶绿体基因标记中，种间差异分别占总变异的16.27%和16.53%，种内差异分别占56.35%和83.47%，个体间差异分别占27.39%和0%。在结构分析中，32个核SSR标记在357个单株中检测到3个亚群，而6个叶绿体基因标记在160个材料中检测到3个亚群。在聚类分析中，3个自交种聚为一类，而近交种则明显分化，特别是在核SSR标记上。此外，几乎所有的多花黑麦草种群都聚为C4群，可进一步分为3个亚群，而多年生黑麦草种群主要聚为两个群(C2和C3)，少数品系与多花黑麦草(C4)或刚性黑麦草(C6)聚为一类。这些研究结果将有助于黑麦草种质资源的改良利用，提高黑麦草育种的有效性。
To assess the genetic diversity and population structure of Lolium species, we used 32 nuclear simple sequence repeat (SSR) markers and 7 cytoplasmic gene markers to analyze a total of 357 individuals from 162 accessions of 9 Lolium species. This survey revealed a high level of polymorphism, with an average number of alleles per locus of 23.59 and 5.29 and an average PIC-value of 0.83 and 0.54 for nuclear SSR markers and cytoplasmic gene markers, respectively. Analysis of molecular variance (AMOVA) revealed that 16.27 and 16.53% of the total variation was due to differences among species, with the remaining 56.35 and 83.47% due to differences within species and 27.39 and 0% due to differences within individuals in 32 nuclear SSR markers set and 6 chloroplast gene markers set, respectively. The 32 nuclear SSR markers detected three subpopulations among 357 individuals, whereas the 6 chloroplast gene markers revealed three subpopulations among 160 accessions in the STRUCTURE analysis. In the clustering analysis, the three inbred species clustered into a single group, whereas the outbreeding species were clearly divided, especially according to nuclear SSR markers. In addition, almost all Lolium multiflorum populations were clustered into group C4, which could be further divided into three subgroups, whereas Lolium perenne populations primarily clustered into two groups (C2 and C3), with a few lines that instead grouped with L. multiflorum (C4) or Lolium rigidum (C6). Together, these results will useful for the use of Lolium germplasm for improvement and increase the effectiveness of ryegrass breeding.