Palladium-Assisted Removal of a Solubilizing Tag from a Cys Side Chain To Facilitate Peptide and Protein Synthesis
Palladium-Assisted Removal of a Solubilizing Tag from a Cys Side Chain To Facilitate Peptide and Protein Synthesis
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DOI：
10.1021/acs.orglett.6b01442
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发表时间：
2016-06-17
期刊：
影响因子：
5.2
通讯作者：
Brik, Ashraf
中科院分区：
文献类型：
--
作者：
Maity, Suman Kumar;Mann, Guy;Brik, Ashraf
在蛋白质化学合成中，将可溶性标签可逆地连接到疏水肽上以促进其纯化和连接是一项重要而又具有挑战性的任务。报道了有效的钯辅助去除与Cys侧链连接的增溶标签。该策略被应用于从两个片段通过连接、去除增溶标签和脱硫的一锅操作高效制备组蛋白H4。
Reversible attachment of solubilizing tags to hydrophobic peptides to facilitate their purification and ligation is an essential yet challenging task in chemical protein synthesis. The efficient palladium-assisted removal of the solubilizing tag linked to the Cys side chain is reported. The strategy was applied for the efficient preparation of histone protein H4 from two fragments via one-pot operation of ligation, removal of the solubilizing tag, and desulfurization.