THE SENSITIVITY OF LYMPHOCYTES TO IONISING RADIATION
THE SENSITIVITY OF LYMPHOCYTES TO IONISING RADIATION
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DOI：
10.1002/path.1700640403
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发表时间：
1952-01-01
期刊：
影响因子：
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通讯作者：
TROWELL, OA
中科院分区：
文献类型：
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作者：
TROWELL, OA
对大鼠淋巴结进行体外培养，并与体内同类细胞进行放射敏感性比较。细胞计数来自膜制备物。给出5小时固缩的淋巴细胞的百分比。在不同剂量的X光照射后根据剂量-效应曲线，ED 50（5小时内产生50%固缩的剂量）体外275 r，体内150 r。淋巴结淋巴细胞的高辐射敏感性可能是细胞本身的特性，而辐射的间接影响很小或没有。中等淋巴细胞与小淋巴细胞一样敏感。网状细胞、单核细胞和浆细胞不受所用剂量的影响。全血体外培养和照射，血淋巴细胞的ED_（50）为1600 r. 400 r全身照射后，大鼠外周血中发现固缩的淋巴细胞，但它们很快被肺部从血流中清除。小肠绒毛内散在淋巴细胞相对抵抗，ED_（50）为2250 r.
The lymph nodes of rats were cultured in vitro and the radiosensitivity of the lymphocytes was compared with that of similar cells in vivo. Cell counts from film prepns. gave the percentage of lymphocytes which were pyknotic 5 hrs. after various doses of X-rays. From the dose-effect curve, the ED50 (dose to produce 50% pyknosis in 5 hrs.) was 275 r in vitro and 150 r in vivo. The high radiosensitivity of lymph-node lymphocytes is probably a property of the cells themselves, while the indirect effects of radiation are slight or nil. Medium lymphocytes were about as sensitive as small lymphocytes. Reticular cells, monocytes and plasma cells were unaffected by the doses used. Whole blood was cultured and irradiated in vitro and the ED50 for blood lymphocytes was 1600 r. Pyknotic lymphocytes were found in the peripheral blood of rats after 400 r whole-body irradiation, but they were rapidly removed from the blood stream by the lungs. The scattered lymphocytes in the intestinal villi were relatively resistant, the ED50 being 2250 r.