Potato extract (Potein) suppresses food intake in rats through inhibition of luminal trypsin activity and direct stimulation of cholecystokinin secretion from enteroendocrine cells.
Potato extract (Potein) suppresses food intake in rats through inhibition of luminal trypsin activity and direct stimulation of cholecystokinin secretion from enteroendocrine cells.
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马铃薯提取物 (Potein) 通过抑制管腔胰蛋白酶活性和直接刺激肠内分泌细胞分泌胆囊收缩素来抑制大鼠的食物摄入。
DOI：
10.1021/jf200988f
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Nakajima S
中科院分区：
文献类型：
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作者：
坂井公紀;坂口和靖;坂井公紀;坂井公紀;坂井公紀;坂井公紀;坂井公紀;坂井公紀;坂井公紀;坂井公紀;Nakajima S;Chen W;Nakajima S
已知膳食蛋白质和胰蛋白酶抑制剂刺激饱腹感激素胆囊收缩素（CCK）的分泌。马铃薯提取物（Potein）含有60%的碳水化合物和20%的蛋白质，包括胰蛋白酶抑制蛋白。在这项研究中，我们研究了Potein是否抑制大鼠的食物摄入，以及它是否直接刺激肠内分泌细胞的CCK分泌。在禁食大鼠中，在口服Potein或大豆胰蛋白酶抑制剂（SBTI）后6 h测量摄食量。在小鼠CCK产生细胞系STC-1中检测Potein和SBTI的CCK释放活性。Potein对胰蛋白酶活性的抑制作用比SBTI低20倍。口服Potein剂量依赖性地抑制食物摄入1-6小时。Potein，而不是SBTI，剂量依赖性地诱导STC-1细胞的CCK分泌。这些结果表明，Potein通过直接刺激肠内分泌细胞诱导CCK分泌和抑制管腔胰蛋白酶来抑制摄食。
Dietary proteins and trypsin inhibitors are known to stimulate the secretion of the satiety hormone cholecystokinin (CCK). A potato extract (Potein) contains 60% carbohydrate and 20% protein including trypsin inhibitory proteins. In this study, we examined whether Potein suppresses food intake in rats and whether it directly stimulates CCK secretion in enteroendocrine cells. In fasted rats, food consumption was measured up to 6 h after the oral administration of Potein or soybean trypsin inhibitor (SBTI). CCK-releasing activities of Potein and SBTI were examined in the murine CCK-producing cell line STC-1. Potein inhibited the trypsin activity in vitro with a potency 20-fold lower than that of SBTI. Oral administration of Potein dose-dependently suppressed food intake for 1–6 h. Potein, but not the SBTI, dose-dependently induced CCK secretion in STC-1 cells. These results suggest that Potein suppresses food intake through the CCK secretion induced by direct stimulation on enteroendocrine cells and through inhibition of luminal trypsin.