Microtubule stabilization opposes the (TNF-alpha)-induced loss in the barrier integrity of corneal endothelium.
Microtubule stabilization opposes the (TNF-alpha)-induced loss in the barrier integrity of corneal endothelium.
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DOI：
10.1016/j.exer.2009.08.004
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发表时间：
2009-12
影响因子：
3.4
通讯作者：
Srinivas, Sangly P.
中科院分区：
文献类型：
--
作者：
Shivanna, Mahesh;Srinivas, Sangly P.
微管分解破坏了许多上皮和内皮单层的屏障完整性。本研究研究了TNF-α对培养的牛角膜内皮细胞微管和屏障完整性的影响，TNF-α与角膜异体移植排斥反应有关。暴露于TNF- α会导致微管的解体，也会导致周围结膜肌动球蛋白环（PAMR）的破坏。跨内皮电阻（TER）是一种测量屏障完整性的方法，基于电细胞-基底阻抗实时检测。暴露于TNF- α可导致TER缓慢下降bbbb20小时，而类似暴露于多孔培养插入物上的细胞可导致对FITC葡聚糖的通透性显著增加。这些变化表明屏障完整性的丧失，也反映在细胞边界ZO-1的错位和钙粘蛋白的拆卸上。微管稳定可能是克服移植排斥反应和葡萄膜炎相关炎症期间（TNF-α）诱导的角膜内皮屏障完整性丧失的一种有效策略。
Microtubule disassembly breaks down the barrier integrity in a number of epithelial and endothelial monolayers. This study has investigated effects of TNF-α, which is implicated in corneal allograft rejection, on microtubules and barrier integrity in cultured bovine corneal endothelial cells. Exposure to TNF- α led to disassembly of the microtubules, and also caused disruption of the perijunctional actomyosin ring (PAMR). As a measure of barrier integrity, trans-endothelial electrical resistance (TER) was determined based on electrical cell-substrate impedance sensing in realtime. Exposure to TNF- α caused a slow decline in TER for > 20 h, and a similar exposure to cells grown on porous culture inserts led to a significant increase in permeability to FITC dextran. These changes, indicating a loss of barrier integrity, were also reflected by dislocation of ZO-1 at the cell border and disassembly of cadherins. These effects of TNF- α were inhibited upon stabilization of microtubules by pre-treatment with paclitaxel or epothilone B. Microtubule stabilization may be a useful strategy to overcome (TNF-α)-induced loss of the barrier integrity of corneal endothelium during inflammation associated with transplant rejection and uveitis.
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影响因子：
--
作者：
GOLDBLUM, SE;DING, XD;CAMPBELLWASHINGTON, J
通讯作者：
CAMPBELLWASHINGTON, J
DOI：
10.1152/ajpgi.00412.2004
发表时间：
2005-03-01
影响因子：
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Ma, TY;Boivin, MA;Said, HM
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发表时间：
2001-06-01
影响因子：
4.9
作者：
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作者：
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DOI：
10.1152/ajplung.00259.2005
发表时间：
2006-03-01
影响因子：
4.9
作者：
Birukova, AA;Adyshev, D;Verin, AD
通讯作者：
Verin, AD