Efficient Conversion of Cane Molasses into Fructooligosaccharides by a Glucose Derepression Mutant of Aureobasidium melanogenum with High beta-Fructofuranosidase Activity
Efficient Conversion of Cane Molasses into Fructooligosaccharides by a Glucose Derepression Mutant of Aureobasidium melanogenum with High beta-Fructofuranosidase Activity
复制标题
通过具有高 β-呋喃果糖苷酶活性的 Aureobasidium melanogenum 葡萄糖去阻抑突变体将甘蔗糖蜜高效转化为低聚果糖
DOI：
10.1021/acs.jafc.9b05826
复制
发表时间：
2019
影响因子：
6.1
通讯作者：
Chi Zhenming
中科院分区：
文献类型：
--
作者：
Zhang Shuhang;Jiang Hong;Xue Sijia;Ge Na;Sun Yi;Chi Zhe;Liu Guanglei;Chi Zhenming
低聚果糖(FOSS)是刺激益生菌的优良食品配料或饲料添加剂。本论文对β-呋喃果糖糖苷酶产生菌33中编码葡萄糖抑制因子的CREA基因进行了干扰，并解除了干扰剂D28中葡萄糖抑制的作用。菌株D28的β-呋喃果糖苷酶活力最高可达2100U/mL，而亲本菌株33和互补菌株C11的产酶活力均低于600U/mL。结果表明，具有较高β-呋喃果糖苷酶活性的葡萄糖去阻遏突变株D28能有效地将工业废糖蜜转化为FOSS。这种低成本、环境友好的生物工艺在生物工程和生物技术生产低聚果糖方面具有巨大的应用潜力。
Fructooligosaccharides (FOSs) are excellent food ingredients or feed additives by stimulating probiotics. In this paper, aCREAgene encoding a glucose repressor in the β-fructofuranosidase producerAureobasidium melanogenum33 with high-level FOS biosynthesis was disrupted, and glucose repression in disruptant D28 was relieved. The disruptant D28 produced up to 2100 U/mL of β-fructofuranosidase activity, whereas the enzyme activities produced by parent strain 33 and complemented strain C11 were below 600 U/mL. The whole cells of the disruptant D28 was used to convert cane molasses into FOSs, and 0.58 g of FOSs/g of molasses sugar was synthesized from 350 g/L cane molasses sugar within 4 h. Results demonstrated that the industrial waste cane molasses can be efficiently converted into FOSs by the glucose derepression mutant D28 with high β-fructofuranosidase activity. This low-cost and environmentally friendly bioprocess has great potential applications in bioengineering and biotechnology for FOS production.