Fur in Magnetospirillum gryphiswaldense influences magnetosomes formation and directly regulates the genes involved in iron and oxygen metabolism.
Fur in Magnetospirillum gryphiswaldense influences magnetosomes formation and directly regulates the genes involved in iron and oxygen metabolism.
复制标题
磁螺菌 gryphiswaldense 中的毛皮影响磁小体的形成并直接调节参与铁和氧代谢的基因
DOI：
10.1371/journal.pone.0029572
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Wu L
中科院分区：
文献类型：
--
作者：
Qi L;Li J;Zhang W;Liu J;Rong C;Li Y;Wu L
Magnetospirillum gryphiswaldense菌株MSR-1具有吸收大量铁并合成磁小体（由Fe3O4组成的细胞内磁性颗粒）的独特能力。 MSR-1 异常高的铁含量使其成为研究铁吸收和稳态生物学机制的有用模型。铁摄取调节剂 (Fur) 蛋白在维持许多细菌的铁稳态方面发挥着关键作用。我们在 MSR-1 中鉴定并鉴定了一个毛皮同源基因 (MGR_1314)。 MGR_1314 能够在体内以铁响应方式补充大肠杆菌的毛皮突变体。我们构建了MSR-1的毛皮突变株。与野生型MSR-1相比，突变菌株磁小体形成较低，对过氧化氢和链黑素更敏感，表明细胞内游离铁含量较高。实时定量RT-PCR和染色质免疫沉淀分析表明，Fur蛋白直接调节参与铁转运和氧代谢的几个关键基因的表达，此外它还在M. gryphiswaldense的磁小体形成中发挥作用。
Magnetospirillum gryphiswaldense strain MSR-1 has the unique capability of taking up large amounts of iron and synthesizing magnetosomes (intracellular magnetic particles composed of Fe3O4). The unusual high iron content of MSR-1 makes it a useful model for studying biological mechanisms of iron uptake and homeostasis. The ferric uptake regulator (Fur) protein plays a key role in maintaining iron homeostasis in many bacteria. We identified and characterized a fur-homologous gene (MGR_1314) in MSR-1. MGR_1314 was able to complement a fur mutant of E. coli in iron-responsive manner in vivo. We constructed a fur mutant strain of MSR-1. In comparison to wild-type MSR-1, the mutant strain had lower magnetosome formation, and was more sensitive to hydrogen peroxide and streptonigrin, indicating higher intracellular free iron content. Quantitative real-time RT-PCR and chromatin immunoprecipitation analyses indicated that Fur protein directly regulates expression of several key genes involved in iron transport and oxygen metabolism, in addition it also functions in magnetosome formation in M. gryphiswaldense.
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影响因子：
3.2
作者：
NIEDERHOFFER, EC;NARANJO, CM;FEE, JA
通讯作者：
FEE, JA
亚铁转运蛋白 B 基因 (feoB1) 在 Magnetospirillum gryphiswaldense 菌株 MSR-1 的磁小体形成中起辅助作用
影响因子：
2.6
作者：
Rong, Chengbo;Huang, Yijun;Li, Jilun
通讯作者：
Li, Jilun
影响因子：
14.9
作者：
Tichopad, A;Dilger, M;Pfaffl, MW
通讯作者：
Pfaffl, MW
影响因子：
3.2
作者：
GREGORY, EM;FRIDOVICH, I
通讯作者：
FRIDOVICH, I
影响因子：
3.2
作者：
KAMMLER, M;SCHON, C;HANTKE, K
通讯作者：
HANTKE, K