Transport of lactate and pyruvate in the intraerythrocytic malaria parasite, Plasmodium falciparum
Transport of lactate and pyruvate in the intraerythrocytic malaria parasite, Plasmodium falciparum
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DOI：
10.1042/bj3550733
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发表时间：
2001-05-01
影响因子：
4.1
通讯作者：
Kirk, K
中科院分区：
文献类型：
--
作者：
Elliott, JL;Saliba, KJ;Kirk, K
成熟的红细胞内形式的人类疟疾寄生虫，恶性疟原虫，是依赖于糖酵解的能量需求。它产生大量的乳酸，必须从寄生虫的胞质溶胶中去除，以保持细胞的完整性和代谢活力。在这里，我们表明，单羧酸乳酸和丙酮酸都通过H+/单羧酸同向转运过程，这是饱和的，并抑制了biformophloretin跨寄生虫的质膜运输。结果提供了直接的证据，在寄生虫表面的H+-偶联单羧酸转运蛋白的功能共同的MCT（单羧酸转运蛋白）家庭的成员，高等真核生物的存在。
The mature, intraerythrocytic form of the human malaria parasite, Plasmodium falciparum, is reliant on glycolysis for its energetic requirements. It produces large quantities of lactic acid, which have to be removed from the parasite's cytosol to maintain the cell's integrity and metabolic viability. Here we show that the monocarboxylates lactate and pyruvate an both transported across the parasite's plasma membrane via a H+/monocarboxylate symport process that is saturable and inhibited by the bioflavonoid phloretin. The results provide direct evidence for the presence at the parasite surface of a H+-coupled monocarboxylate transporter with features in common with members of the MCT (monocarboxylate transporter) family of higher eukaryotes.