Potential role of 11 beta-hydroxysteroid dehydrogenase in human trophoblast-endometrial interactions.
Potential role of 11 beta-hydroxysteroid dehydrogenase in human trophoblast-endometrial interactions.
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11β-羟基类固醇脱氢酶在人类滋养层-子宫内膜相互作用中的潜在作用。
DOI：
10.1111/j.1749-6632.1996.tb16258.x
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发表时间：
1996
影响因子：
5.2
通讯作者：
Schatz,F
中科院分区：
文献类型：
--
作者：
Arcuri,F;Monder,C;Battistini,S;Hausknecht,V;Lockwood,CJ;Schatz,F
对肾上腺灌注的研究结果，结合对正常人2以及患有库欣病的人的尿液分析，确定皮质醇和皮质酮是肾上腺的主要分泌产物。随后，在大鼠肝脏中发现了一种能够将皮质醇氧化为可的松的酶。4命名为11 p-HSD，发现其可逆地将皮质醇和皮质酮转化为它们的无活性的11-氧化对，可的松和11-脱氢皮质酮。现在已经在多种组织中检测到llp-HSD的存在。虽然11 β-HSD活性主要定位于肝、肾、胎盘和脾的微粒体中，但在肾和脑的细胞核中也有显著的活性。“此外，肾微粒体和核11 P-HSD活性的K值相似，表明酶是相同或相似的。令人惊讶的是，对于Ila-HSD活性最佳的K和pH的报道值在组织与组织之间的异常宽的范围内变化，并且对于相同的组织，它们在实验室与实验室之间的变化甚至更大。Monder和WhiteI 2列出了底物和辅因子浓度、pH值和孵育温度的变异性，以解释缺乏重现性的原因，并特别强调了研究者之间组织制备方法的差异。
The results of studies with perfused adrenals,'taken together with analysis of urine from normal individuals, 2 as well as those suffering from Cushing's di~ ease,~ identified cortisol and corticosterone as the predominant secretory products of the adrenal gland. Subsequently, an enzyme capable of oxidizing cortisol to cortisone was reported in rat liver. 4 Given the name 1 lp-HSD, it was found to reversibly convert cortisol and corticosterone to their inactive 1 1-oxygenated pairs, cortisone and ll-dehydrocorticosterone. 5 The presence of l lp-HSD has now been detected in diverse tissues. Although 11p-HSD activity has been localized primarily in the microsomes of liver: kidney, l placenta,'and~ pleen,~ significant activity is also reported in nuclei of kidneylo and brain." Moreover, the similarity in K, values for kidney microsomal and nuclear 11P-HSD activityI0 suggests that the enzymes are identical or similar. Surprisingly, reported values of K, and pH that are optimal for 1 la-HSD activity vary over an unusually wide range from tissue to tissue and they vary even more widely from laboratory to laboratory for the same tissue. Monder and WhiteI2 listed variability in substrate and cofactor concentrations, pH, and temperature of incubation to account for this lack of reproducibility and gave special emphasis to differences in methods of tissue preparations among investigators.