A human protein with homology to Saccharomyces cerevisiae SNF5 interacts with the potential helicase hbrm.
A human protein with homology to Saccharomyces cerevisiae SNF5 interacts with the potential helicase hbrm.
复制标题
与酿酒酵母 SNF5 同源的人类蛋白质与潜在的解旋酶 hbrm 相互作用。
DOI：
10.1093/nar/23.7.1127
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发表时间：
1995
影响因子：
14.9
通讯作者：
Yaniv,M
中科院分区：
文献类型：
--
作者：
Muchardt,C;Sardet,C;Bourachot,B;Onufryk,C;Yaniv,M
在酵母中，SNF/SWI复合体参与了几个可诱导启动子的转录激活，可能是通过引起染色质结构的局部修饰。最近，已经分离出SNF2/SWI2蛋白的两个人类同源物，hbrm和Bag-1。此外，还从HeLa细胞中部分纯化了一个含有SNF2/SWI2同系物的复合体，该复合体具有与酵母复合体相似的Anin体外活性。在这里，我们描述了一个编码人核蛋白的cDNA的特征，该蛋白包含一个与SNF5同源的大区域，SNF5是酵母SNF/SW1复合体的另一个成员。这种蛋白可以与hbrm免疫共沉淀，两种蛋白之间的相互作用依赖于人和酵母SNF5之间的保守区域。这些发现表明，我们克隆的cDNA编码人SNF/SWI复合体的一个成员。
ABSTRACTIn yeast, the SNF/SWI complex is involved in transcriptional activation of several inducible promoters, possiblyby causing a local modification of the chromatin structure. Recently, two human homologues of the SNF2/SWI2 proteinhave been isolated, hbrm and BAG-1. In addition, a complex containing one of the SNF2/SWI2 homologues and having anIn vitroactivity similar to the yeast complex has been partially purified from HeLa cells. Here we describe the characterization of a cDNA encoding a human nuclear protein containing a large domain of homology with SNF5, another member of the yeast SNF/SW1 complex. This protein can be co-immunoprecipitated with hbrm and the interaction between the two proteins is dependent on the region conserved between the human and the yeast SNF5. These findings suggest that the cDNA we have cloned encodes one of the members of the human SNF/SWI complex.