Genome-wide association mapping identifies markers associated with cane yield components and sucrose traits in the Louisiana sugarcane core collection
Genome-wide association mapping identifies markers associated with cane yield components and sucrose traits in the Louisiana sugarcane core collection
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DOI：
10.1016/j.ygeno.2018.12.002
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发表时间：
2019-12-01
期刊：
影响因子：
4.4
通讯作者：
Baisakh, Niranjan
中科院分区：
文献类型：
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作者：
Fickett, Nathanael;Gutierrez, Andres;Baisakh, Niranjan
甘蔗是粮食和生物燃料工业的重要经济作物。随着抗病基因标记的开发和应用，甘蔗分子标记辅助育种已成为现实。甘蔗的大连锁不平衡使全基因组关联研究（GWAS）成为双亲作图的一个更好的选择，以确定与农艺性状相关的标记。利用单核苷酸多态性（SNP）和插入-缺失（Indel）标记对路易斯安那州甘蔗核心种质的11个甘蔗产量和蔗糖产量性状进行了GWAS分析。对于所有性状鉴定了显著（P <0.05）的MTA，其中排名靠前的标记解释了高达15%的总表型变异。蔗糖品质性状间的相关系数在0.732~0.999之间，共检测到56个标记。这些标记在更多样化的群体中验证后，可用于路易斯安那州和其他地方甘蔗育种计划中的标记辅助选择无性系。
Sugarcane is an economically important crop for both food and biofuel industries. Marker-assisted breeding in sugarcane is becoming a reality with the recent development and deployment of markers linked with disease resistance genes. Large linkage disequilibrium in sugarcane makes genome-wide association studies (GWAS) a better alternative to biparental mapping to identify markers associated with agronomic traits. GWAS was conducted on a Louisiana core collection to identify marker-trait associations (MTA) for 11 cane yield and sucrose traits using single nucleotide polymorphism (SNP) and insertion-deletion (Indel) markers. Significant (P < .05) MTAs were identified for all traits where the top ranked markers explained up to 15% of the total phenotypic variation. High correlations (0.732 to 0.999) were observed between sucrose traits and 56 markers were found consistent across multiple traits. These markers following validation in more diverse populations could be used in marker-assisted selection of clones in sugarcane breeding program in Louisiana and elsewhere.