Systematic evaluation of FRAP experiments performed in a confocal laser scanning microscope - Part II: Multiple diffusion processes
Systematic evaluation of FRAP experiments performed in a confocal laser scanning microscope - Part II: Multiple diffusion processes
复制标题
DOI：
10.1111/j.1365-2818.2008.01993.x
复制
发表时间：
2008-06-01
影响因子：
2
通讯作者：
Oppermann, W.
中科院分区：
文献类型：
--
作者：
Hauser, G. I.;Seiffert, S.;Oppermann, W.
提出了一种用于系统评估光漂白后荧光恢复(FRAP)数据的程序，该程序允许确定扩散系数的分布。当激光扫描共聚焦显微镜测量FRAP随时间和空间的变化时，该方法提供了一种直接且无需校准的方法来量化多个扩散过程。利用不同尺寸荧光微球混合物的FRAP实验验证了这是离散D分布的实现。
A procedure for a systematic evaluation of fluorescence recovery after photo-bleaching (FRAP) data is presented that allows one to determine distributions of diffusion coefficients. The method provides a straightforward and calibration-free way to quantify multiple diffusion processes when FRAP is measured as a function of time and space by means of confocal laser scanning microscopy. It is verified utilizing FRAP experiments on mixtures of differently sized fluorescent micro-spheres as realizations of discrete D distributions.