CROSS-REACTIVITY OF MONOCLONAL-ANTIBODIES AGAINST CLOSTRIDIUM-PERFRINGENS THETA-TOXIN WITH STREPTOLYSIN-O
CROSS-REACTIVITY OF MONOCLONAL-ANTIBODIES AGAINST CLOSTRIDIUM-PERFRINGENS THETA-TOXIN WITH STREPTOLYSIN-O
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DOI：
10.1007/bf01577135
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发表时间：
1984-01-01
影响因子：
2.6
通讯作者：
CHIBA, J
中科院分区：
文献类型：
--
作者：
SATO, H;ITO, A;CHIBA, J
六种小鼠单克隆抗体。对产气荚膜荚膜杆菌毒素进行了表征。四种抗体IC3、2D4、1B9和3F11对. theta不中和。与溶血素O （SLO）无交叉反应。另外2个抗体3H10和2C5与SLO交叉结合和交叉中和。从与。theta的结合活性来看，3H10的中和活性高于2C5。毒素和SLO。两种抗体均能抑制毒素与绵羊红细胞结合后的溶血，抑制毒素对培养心脏细胞的心脏毒性。
Six mouse monoclonal antibodies to .THETA. toxin of C. perfringens were characterized. Four antibodies, IC3, 2D4, 1B9 and 3F11, were nonneutralizing for .THETA. toxin and were non-cross-reacting with streptolysin O (SLO). The other 2 antibodies, 3H10 and 2C5, were cross-binding and cross-neutralizing with SLO. Neutralizing activity of 3H10 was higher than that of 2C5 on the basis of the binding activity with .THETA. toxin and SLO. Both antibodies could inhibit hemolysis even after binding of the toxins to sheep red blood cells and inhibited cardiotoxicity of the toxins in cultured heart cells.