Electrochemical desorption of self-assembled monolayers noninvasively releases patterned cells from geometrical confinements
Electrochemical desorption of self-assembled monolayers noninvasively releases patterned cells from geometrical confinements
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DOI：
10.1021/ja029485c
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发表时间：
2003-03-05
影响因子：
15
通讯作者：
Whitesides, GM
中科院分区：
文献类型：
--
作者：
Jiang, XY;Ferrigno, R;Whitesides, GM
本报告描述了一种方法，以图案哺乳动物细胞使用自组装单分子膜（SAMs），然后使用这些单分子膜的电化学解吸释放细胞从他们的模式。该方法使用寡聚（乙二醇）封端的SAM来防止，并使用甲基封端的SAM来允许蛋白质的吸附和牛毛细血管内皮细胞的附着。电化学去除的寡聚（乙二醇）-终止SAM允许蛋白质吸附到以前是惰性的区域，并使细胞迁移到这些地区。这种简单的技术在药物筛选的生物测定和细胞生物学的基础研究中是有用的。
This report describes a method to pattern mammalian cells using self-assembled monolayers (SAMs), and then to use electrochemical desorption of these monolayers to release cells from their patterns. This method uses an oligo(ethyleneglycol)-terminated SAM to prevent,and a methyl-terminated SAM to allowadsorption of proteins and attachment of bovine capillary endothelial cells. Electrochemical removal of the oligo(ethyleneglycol)-terminated SAM allowed proteins to adsorb onto areas that had been previously inert and enabled cells to migrate into these areas. This straightforward technique is useful in bioassays for drug screening and for fundamental studies in cell biology.