Transcriptome Complexity in a Genome-Reduced Bacterium
Transcriptome Complexity in a Genome-Reduced Bacterium
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DOI：
10.1126/science.1176951
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发表时间：
2009-11-27
期刊：
影响因子：
56.9
通讯作者：
Serrano, Luis
中科院分区：
文献类型：
--
作者：
Gueell, Marc;van Noort, Vera;Serrano, Luis
为了研究细菌中转录组组织的基本原理，我们分析了最小的自我复制生物之一，肺炎支原体。我们结合了链特异性平铺阵列，辅以转录组测序，超过252点阵列。我们检测到117个以前未描述的，主要是非编码转录，其中89个在反义配置已知的基因。我们确定了341个操纵子，其中139个是多顺反子，后者几乎一半的表现出衰退的表达在一个阶梯状的方式。在不同的条件下，操纵子可以被分成447个更小的转录单位，产生许多不同的转录本。每个基因频繁的反义转录本、替代转录本和多个调节子意味着高度动态的转录组，比之前认为的更类似于真核生物。
To study basic principles of transcriptome organization in bacteria, we analyzed one of the smallest self-replicating organisms, Mycoplasma pneumoniae. We combined strand-specific tiling arrays, complemented by transcriptome sequencing, with more than 252 spotted arrays. We detected 117 previously undescribed, mostly noncoding transcripts, 89 of them in antisense configuration to known genes. We identified 341 operons, of which 139 are polycistronic; almost half of the latter show decaying expression in a staircase-like manner. Under various conditions, operons could be divided into 447 smaller transcriptional units, resulting in many alternative transcripts. Frequent antisense transcripts, alternative transcripts, and multiple regulators per gene imply a highly dynamic transcriptome, more similar to that of eukaryotes than previously thought.