Correlated asymmetry of sequence and functional divergence between duplicate proteins of saccharomyces cerevisiae
Correlated asymmetry of sequence and functional divergence between duplicate proteins of saccharomyces cerevisiae
复制标题
DOI：
10.1093/molbev/msj115
复制
发表时间：
2006-05-01
影响因子：
10.7
通讯作者：
Yi, SV
中科院分区：
文献类型：
--
作者：
Kim, SH;Yi, SV
序列差异在重复基因功能差异中的作用是人们非常感兴趣的话题。在这项研究中，我们使用一个复制前的祖先外群，比较了自从两个酵母复制分叉以来，每个序列中氨基酸替换的数量。使用这一策略，我们探索了重复伙伴之间的序列差异和功能差异之间的关系。我们发现，重复蛋白质之间的相对功能不对称程度与它们之间的相对序列差异成正比。此外，在两个重复的副本中，更接近其祖先序列的副本(氨基酸替换的数量较少)与更多的蛋白质相互作用，当被删除时，对适合性的影响更严重。因此，复制体之间的不对称序列差异与不对称功能差异相关，并可能是复制体对突变的遗传稳健性作用的基础。在所考虑的功能特征中，蛋白质丰度似乎与复制品之间的非同义差异具有最强的相关性。结合全基因组分析的结果，我们的结果表明，物种内的复制受到与物种间序列和功能差异相同的进化力的影响。特别是，我们在进化较慢的副本上检测到净化选择的迹象。
The role of sequence divergence in functional divergence of duplicate genes is a topic of great interest. In this study, we compare the numbers of amino acid substitutions in each sequence since two yeast duplicates diverged, using a preduplication ancestral outgroup. Using this strategy, we explored the relationship between sequence divergence and functional divergence between duplicate partners. We show that the degree of relative functional asymmetry between duplicate proteins is proportional to the relative sequence divergence between them. Furthermore, of the two duplicates, the copy closer to their ancestral sequence (fewer number of amino acid substitutions) interacts with more proteins and affects fitness more severely when deleted. Therefore, asymmetric sequence divergence between duplicates is correlated with asymmetric functional divergence and may underlie the duplicate's role in genetic robustness against mutations. Among the functional traits considered, protein abundance appears to have the strongest correlation with the nonsynonymous divergence between duplicates. Taken together with the results from whole-genome analyses, our results indicate that within-species duplicates are subject to the same evolutionary force that acts on interspecific sequence and functional divergence. In particular, we detect signs of purifying selection on the more slowly evolving duplicate.