Ocular conjunctival inoculation of SARS-CoV-2 can cause mild COVID-19 in rhesus macaques.
Ocular conjunctival inoculation of SARS-CoV-2 can cause mild COVID-19 in rhesus macaques.
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DOI：
10.1038/s41467-020-18149-6
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发表时间：
2020-09-02
影响因子：
16.6
通讯作者：
Qin, Chuan
中科院分区：
文献类型：
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作者：
Deng, Wei;Bao, Linlin;Qin, Chuan
严重急性呼吸综合征冠状病毒2(SARS-CoV-2)通过呼吸道途径高度传播，但SARS-CoV-2潜在的呼吸道外传播途径仍不确定。在这里，我们用SARS-CoV-2的1×106 TCID50经结膜(CJ)、气管内(IT)和胃内(IG)接种5只恒河猴。从CJ和IT采集的鼻咽拭子在接种后1-7天(Dpi)检测到病毒RNA。仅在1-7dpi的IT组的肛拭子中检测到病毒RNA。灌胃接种后的拭子和组织中未检测到病毒RNA。感染CJ的动物鼻泪系统的病毒载量高于IT感染的动物，但也表现出轻微的间质性肺炎，表明病毒分布不同。这项研究表明，通过结膜途径感染在非人类灵长类动物中是可能的；需要进一步的研究来更详细地比较通过这些不同途径感染的相对风险和发病机制。
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly transmitted through the respiratory route, but potential extra-respiratory routes of SARS-CoV-2 transmission remain uncertain. Here we inoculated five rhesus macaques with 1*106 TCID50 of SARS-CoV-2 conjunctivally (CJ), intratracheally (IT), and intragastrically (IG). Nasal and throat swabs collected from CJ and IT had detectable viral RNA at 1-7 days post-inoculation (dpi). Viral RNA was detected in anal swabs from onlythe IT group at 1-7 dpi. Viral RNA was undetectable in tested swabs and tissues after intragastric inoculation. The CJ infected animal had a higher viral load in the nasolacrimal system than the IT infected animal but also showed mild interstitial pneumonia, suggesting distinct virus distributions. This study shows that infection via the conjunctival route is possible in non-human primates; further studies are necessary to compare the relative risk and pathogenesis of infection through these different routes in more detail.