Small intestinal morphology and bacterial populations in ileal digesta and feces of newly weaned pigs receiving a high dietary level of zinc oxide
Small intestinal morphology and bacterial populations in ileal digesta and feces of newly weaned pigs receiving a high dietary level of zinc oxide
复制标题
DOI：
10.4141/a01-043
复制
发表时间：
2001-12-01
影响因子：
1
通讯作者：
Kirkwood, RN
中科院分区：
文献类型：
--
作者：
Li, BT;Van Kessel, AG;Kirkwood, RN
本试验研究了日粮中添加3000 mg kg（-1）氧化锌（ZnO）对断奶仔猪小肠形态及回肠和粪便中肠杆菌科、乳酸杆菌和梭菌数量的影响。在17日龄时，来自9窝的36头猪在回肠远端安装了简单的T型插管，术后2小时恢复后返回母猪。在21日龄时，将猪断奶并圈养在单独的代谢箱中。猪被分配接受标准的起始饲料补充或不补充3000毫克公斤（-1）的氧化锌。在断奶前即刻和断奶后第2、4、7、9和11天收集比尔粪便和粪便样本，并通过选择性培养基上的菌落计数用于定量肠杆菌科、乳酸杆菌和梭菌种群。在最后一次取样后对猪实施安乐死，并从沿小肠长度沿着的25、50和75%的部位收集2 cm组织切片，用于测定粘膜厚度（MT）、隐窝深度（CD）、绒毛高度（VH）和绒毛宽度（VW）。补充氧化锌改变了小肠的粘膜形态。添加ZnO后，小肠长度25%和50%沿着的粘膜厚度（P < 0.08）和VH（P < 0.05）增加。添加ZnO后，VW也显著增加（P < 0.01）。在小肠长度方向上，小肠隐窝深度下降了沿着75%（P < 0.05）。在小肠长度的25、50和75%沿着处，补充ZnO的猪的VH与CD的比率高于对照组（P < 0.05）。添加氧化锌对回肠和粪便中细菌数量无影响（P > 0.05）。本研究表明，在仔猪日粮中添加氧化锌会改变小肠上皮细胞的形态，从而影响仔猪对营养物质的消化吸收。
A study was carried out to determine the effect of dietary supplementation of 3000 mg kg(-1) zinc oxide (ZnO) on the small intestinal morphology and populations of enterobacteriaceae, lactobacilli and clostridia in ileal digesta and feces of weaned pigs. At 17 d of age, 36 pigs from nine litters were fitted with simple T-cannulae at the distal ileum and after a 2-h post-surgery recovery returned to their sows. At 21 d of age, the pigs were weaned and housed in individual metabolism crates. Pigs were allocated to receive a standard starter diet supplemented with or without 3000 mg kg(-1) of ZnO. Beal digesta and fecal samples were collected immediately before weaning and then on days 2, 4, 7, 9, and 11 after weaning and were used to quantify enterobacteriaceae, lactobacilli and clostridial populations by colony enumeration on selective media. Pigs were euthanized following the final sampling, and 2cm sections of tissue were collected from sites 25, 50 and 75% along the length of the small intestine for determination of mucosal thickness (MT), crypt depth (CD), villous height (VH) and villous width (VW). Zinc oxide supplementation altered the mucosal morphology of the small intestine. Mucosal thickness (P < 0.08) and VH (P < 0.05) were increased at sites 25 and 50% along the length of the small intestine by ZnO supplementation. Overall VW also increased (P < 0.01) with ZnO supplementation. Crypt depth decreased (P < 0.05) at 75% along the length of the small intestine with ZnO supplementation. The ratio of VH to CD was higher (P < 0.05) for ZnO-supplemented than for control-fed pigs at sites 25, 50 and 75% along the length of the small intestine. There was no effect (P > 0.05) of supplementary ZnO on bacterial populations in ileal digesta or feces. The present study indicates that supplementing ZnO in starter diets changes the epithelial morphology of the small intestine, which may affect nutrient digestion and absorption in newly weaned pigs.