Prevention of denervated muscle atrophy with accelerated nerve-regeneration by babysitter procedure in rat facial nerve paralysis model
Prevention of denervated muscle atrophy with accelerated nerve-regeneration by babysitter procedure in rat facial nerve paralysis model
复制标题
在大鼠面神经麻痹模型中通过保姆程序加速神经再生来预防失神经肌肉萎缩
DOI：
10.1002/micr.30580
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发表时间：
2020
期刊：
影响因子：
2.1
通讯作者：
Sakurai Hiroyuki
中科院分区：
文献类型：
--
作者：
Hashimoto Kazuki;Matsumine Hajime;Osaki Hironobu;Ueta Yoshifumi;Kamei Wataru;Shimizu Mari;Fujii Kaori;Niimi Yosuke;Miyata Mariko;Sakurai Hiroyuki
目的“保姆”手术是一种利用健康面神经的运动源和交叉神经移植治疗面神经完全麻痹的方法。首先，在受影响的面神经干和舌下神经之间进行端侧神经吻合，持续向模拟肌肉传递刺激，防止模拟肌肉萎缩。尽管临床结果良好，但组织学和生理机制尚不清楚。本研究试图建立一个“保姆”程序的模型，并观察其在面神经完全麻痹大鼠中的疗效。材料与方法选用Lewis大鼠16只，分为2组；交叉神经移植组（n= 8）和保姆组（n= 8）。两组均行面神经干横切术。保姆组接受了两个阶段的程序。交叉神经移植物组仅将神经移植物从健康侧转移到患侧。术后13周，采用复合肌动作电位（CMAP）对动物进行生理评估，并对再生神经组织进行组织病理学评估。结果用逆行示踪剂对面神经核进行染色，证实了保姆法对面肌的再神经支配。在CMAP中，保姆组的振幅显著高于面神经移植物组（p< 0.05）。组织学检查发现两组间髓磷脂g -比值有显著差异（p< 0.05）。结论本研究探讨了大鼠面神经麻痹的“保姆”治疗方法。保姆手术缩短了去神经期，但没有出现肌肉萎缩。
PurposeThe “babysitter” procedure is a reconstruction technique for facial nerve complete paralysis and uses the movement source from the healthy facial nerve with a cross‐nerve graft. First, an end‐to‐side neurorrhaphy is performed between the affected facial nerve trunk and hypoglossal nerve for continuously delivering stimuli to the mimetic muscles for preventing the atrophy of mimetic muscles. Despite favorable clinical results, histological and physiological mechanisms remain unknown. This study attempted to establish a model for the “babysitter” procedure and find its efficacy in rats with facial nerve complete paralysis.Materials and MethodsA total of 16 Lewis rats were used and divided into 2 groups; cross nerve graft (n= 8) and babysitter groups (n= 8). The facial nerve trunk was transected in both groups. Babysitter group underwent a two‐stage procedure. Cross nerve graft group underwent only the transfer of nerve graft from the healthy side to affected side. The animals were assessed physiologically by compound muscle action potential (CMAP), and the regenerated nerve tissues were evaluated histopathologically at 13 weeks after surgery.ResultsFacial nucleus stained with retrograde tracers proved the re‐innervation of affected facial muscle by the babysitter procedure. In CMAP, the amplitude of babysitter group was significantly higher than that of the cross‐facial nerve graft group (p< .05). Histological examination found a significant difference in myelin g‐ratio between two groups (p< .05).ConclusionThis study investigated the “babysitter” procedure for rat facial nerve palsy. Babysitter procedure shortened the denervation period without mimic muscle atrophy.