AUTOMATED ENZYMATIC ASSAYS FOR THE DETERMINATION OF INTESTINAL PERMEABILITY PROBES IN URINE .2. MANNITOL
AUTOMATED ENZYMATIC ASSAYS FOR THE DETERMINATION OF INTESTINAL PERMEABILITY PROBES IN URINE .2. MANNITOL
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DOI：
10.1016/0009-8981(89)90332-x
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发表时间：
1989-08-15
影响因子：
5
通讯作者：
NORTHROP, AJ
中科院分区：
文献类型：
--
作者：
LUNN, PG;NORTHROP, CA;NORTHROP, AJ
由于在肠道渗透性测试中使用这种单糖，因此需要一种简单的方法来测定尿液中的甘露醇。提出了一种基于细菌酶甘露醇脱氢酶的快速分光光度测定法。该技术已实现自动化，可在 Cobas-Bio（罗氏）离心分析仪上使用。该测定已被证明对甘露醇具有高度特异性，并且不受样品中高浓度葡萄糖、乳糖或乳果糖的影响。测定内变异系数范围为 0.3% 至 1.1%，测定间变异系数范围为 0.6% 至 2.4%。该技术在时间、简单性和精度方面比现有方法有了显着改进。
A need for a simple method for the determination of mannitol in urine has arisen because of the use of this monosaccharide in intestinal permeability tests. A rapid spectrophotometric assay for mannitol is presented based on the use of the bacterial enzyme mannitol dehydrogenase. The technique has been automated for use on the Cobas-Bio (Roche) centrifigal analyser. The assay has been shown to be highly specific for mannitol and was not affected by high concentrations of glucose, lactose or lactulose in samples. Within assay coefficient of variation was in the range 0.3 to 1.1% and 0.6 to 2.4% between assays. The technique represents a significant improvement in terms of time, simplicity and precision on existing methods.