Nuclear respiratory factor 2 activates transcription of human mitochondrial translation initiation factor 2 gene
Nuclear respiratory factor 2 activates transcription of human mitochondrial translation initiation factor 2 gene
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DOI：
10.1016/j.mito.2006.10.005
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发表时间：
2007-05-01
期刊：
影响因子：
4.4
通讯作者：
Takeuchi, Nono
中科院分区：
文献类型：
--
作者：
Hayashi, Rippei;Ueda, Takuya;Takeuchi, Nono
我们研究了人线粒体翻译起始因子2（IF 2 mt）基因的转录调控。人IF 2 mt基因的最小启动子区含有核呼吸因子2（NRF-2）的结合位点，其通常参与泌尿系统相关基因的转录。电泳迁移率变动分析（EMSA）分析表明NRF-2 α/β与IF 2 mt启动子结合。报告基因测定，其中HEK 293 T细胞与NRF-2 α/β表达载体和/或IF 2 mt启动子报告基因载体共转染，揭示NRF-2反式激活IF 2 mt启动子。在其他几种线粒体翻译因子的启动子中也发现了NRF-2位点，这表明NRF-2可能在线粒体蛋白质合成的调控中起关键作用。(c)2006 Elsevier B. V.和线粒体研究学会。All rights reserved.
We studied the transcriptional regulation of the human mitochondrial translation initiation factor 2 (IF2mt) gene. The minimal promoter region for the human IF2mt gene contains binding sites for Nuclear Respiratory Factor 2 (NRF-2), which is often involved in the transcription of mitochondrial-related genes. Electrophoresis mobility shift assay (EMSA) analyses indicated that NRF-2 alpha/beta binds to the IF2mt promoter. Reporter assays, where HEK293T cells were co-transfected with an NRF-2 alpha/beta-expressing vector and/or an IF2mt promoter reporter vector, revealed that NRF-2 trans-activates the IF2mt promoter. NRF-2 sites were also found in the promoters of several other mitochondrial translation factors, which suggests NRF-2 may play a key role in the regulation of mitochondrial protein synthesis. (c) 2006 Elsevier B.V. and Mitochondria Research Society. All rights reserved.