Decreased IL-10-producing regulatory B cells in patients with advanced mycosis fungoides
Decreased IL-10-producing regulatory B cells in patients with advanced mycosis fungoides
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DOI：
10.1684/ejd.2018.3319
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发表时间：
2018-05-01
影响因子：
2.5
通讯作者：
Sato, Shinichi
中科院分区：
文献类型：
--
作者：
Akatsuka, Taro;Miyagaki, Tomomitsu;Sato, Shinichi
背景：历史上，B细胞被认为是体液免疫应答的正调节因子。然而，特定的B细胞亚群负调节免疫应答，并被称为“调节性B细胞”（Bcells）。近年来，研究发现，BAE不仅与炎症和自身免疫性疾病有关，而且通过抑制抗肿瘤免疫与恶性肿瘤有关。目的：探讨晚期蕈样肉芽肿（MF）中是否存在肉毒杆菌。材料和方法：采用流式细胞术检测了11例晚期MF患者和9例健康对照者外周血中CD 19（+）CD 24（hi）CD 27（+）记忆B细胞和CD 19（+）CD 24（hi）CD 38（hi）过渡型B细胞（富含产生IL-10的B细胞）的频率。还通过流式细胞术测量了产生IL-10的BclB的频率。还分析了B细胞亚群的频率或数量与疾病严重程度标志物之间的相关性。结果如下：晚期MF患者外周血中CD 19（+）CD 24（hi）CD 27（+）B细胞、CD 19（+）CD 24（hi）CD 38（hi）B细胞和IL-10产生B细胞的频率降低。这些B细胞亚群的频率和数量与血清可溶性IL-2受体和血清乳酸脱氢酶水平呈负相关。结论：在晚期MF患者中，产生IL-10的β细胞的发育受损，并且产生IL-10的β细胞的减少可能在晚期MF的进展中起重要作用。
Background: Historically, B cells have been considered as positive regulators of humoral immune responses. Specific B-cell subsets, however, negatively regulate immune responses and are termed "regulatory B cells" (Bregs). Recently, Bregs have been linked to not only inflammatory and autoimmune diseases, but also malignancies via suppressing antitumour immunity. Objectives: To investigate the involvement of Bregs in advanced mycosis fungoides (MF). Materials & Methods: The frequency of CD19(+)CD24(hi)CD27(+) memory B cells and CD19(+)CD24(hi)CD38(hi) transitional B cells (which enrich IL-10-producing Bregs) was examined in peripheral blood from patients with advanced MF (n = 11) and healthy controls (n = 9) by flow cytometry. The frequency of IL-10-producing Bregs was also measured by flow cytometry. The correlation between frequency or number of B-cell subsets and disease severity markers was also analysed. Results: The frequency of CD19(+)CD24(hi)CD27(+) B cells, CD19(+)CD24(hi)CD38(hi) B cells, and IL-10-producing B cells was decreased in peripheral blood of advanced MF patients. The frequency and number of these B-cell subsets inversely correlated with serum soluble IL-2 receptor and serum lactate dehydrogenase levels. Conclusions: The development of IL-10-producing Bregs is impaired in patients with advanced MF and a decrease in IL-10-producing Bregs may play an important role in the progression of advanced MF.