Two DM domain genes, DMY and DMRT1, involved in testicular differentiation and development in the medaka, Oryzias latipes
Two DM domain genes, DMY and DMRT1, involved in testicular differentiation and development in the medaka, Oryzias latipes
复制标题
DOI：
10.1002/dvdy.20158
复制
发表时间：
2004-11-01
影响因子：
2.5
通讯作者：
Nagahama, Y
中科院分区：
文献类型：
--
作者：
Kobayashi, T;Matsuda, M;Nagahama, Y
DMY基因是青鳉（Oryzias latipes）Y染色体上的DM结构域基因，也是DMRT 1家族基因之一，是青鳉雄性发育的关键决定基因，是青鳉性别决定的主要候选基因。本研究旨在研究DMY和DMRT 1基因在青鳉性腺分化过程中的表达位点和表达模式，以进一步确定其在精巢发育中的作用。DMY mRNA和蛋白质在早期性腺原基的原始生殖细胞（PGCs）周围的体细胞中特异性表达，然后观察到形态性别差异。然而，周围的体细胞PGCs从来没有表达DMY在早期迁移期。DMY的表达持续存在于支持细胞系细胞中，从PGC支持细胞到支持细胞，表明只有DMY阳性细胞在孵化后的有丝分裂停滞期间包围PGC。DMRT 1在睾丸分化后（孵化后20-30天）的精原支持细胞中表达，其在成熟睾丸中的表达远高于DMY。在XX性反转睾丸中，DMRT 1在支持细胞谱系中表达，类似于XY睾丸中DMY的表达。这些结果强烈表明，DMY调节PGC增殖和分化的性别特异性在早期性腺分化的XY个人和DMRT 1调节精原细胞分化。(C)2004 Wiley-Liss，Inc.
The recent discovery of the DMY gene (DM domain gene on Y chromosome and one of the DMRT1 family genes) as a key determinant of male development in the medaka (Oryzias latipes) has led to its designation as the prime candidate gene for sex-determination in this species. This study focused on the sites and pattern of expression of DMY and DMRT1 genes during gonadal differentiation of medaka to further determine their roles in testis development. DMY mRNA and protein are expressed specifically in the somatic cells surrounding primordial germ cells (PGCs) in the early gonadal primordium, before morphological sex differences are seen. However, somatic cells surrounding PGCs never express DMY during the early migratory period. Expression of DMY persists in Sertoli cell lineage cells, from PGC-supporting cells to Sertoli cells, indicating that only DMY-positive cells enclose PGCs during mitotic arrest after hatching. DMRT1 is expressed in spermatogonium-supporting cells after testicular differentiation (20-30 days after hatching), and its expression is much higher than that of DMY in mature testes. In XX sex-reversed testes, DMRT1 is expressed in the Sertoli cell lineage, similar to the expression of DMY in XY testes. These results suggest strongly that DMY regulates PGC proliferation and differentiation sex-specifically during early gonadal differentiation of XY individuals and that DMRT1 regulates spermatogonial differentiation. (C) 2004 Wiley-Liss, Inc.