Determination of tobramycin in serum using liquid chromatography-tandem mass spectrometry and comparison with a fluorescence polarisation assay
Determination of tobramycin in serum using liquid chromatography-tandem mass spectrometry and comparison with a fluorescence polarisation assay
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DOI：
10.1016/s1570-0232(03)00492-6
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发表时间：
2003-09-05
影响因子：
3
通讯作者：
Cooper, DR
中科院分区：
文献类型：
--
作者：
Keevil, BG;Lockhart, SJ;Cooper, DR
我们已经开发了一种串联质谱（LC-MS-MS）方法测量血清样品中的妥布霉素浓度，并将其与荧光偏振免疫分析法进行了比较。用乙腈沉淀蛋白质后，将上清液进样到LC-MS-MS系统中。用含有HFBA的20-100%甲醇的阶梯梯度洗脱C-18柱（4 x 2 mm）。保留时间为妥布霉素1.05 min和西索霉素1.05 min。MRM转换为：m/z 467.8 > 163（妥布霉素）和m/z 447.8 > 160（西索霉素）。定量限为0.15 mg/l，测定线性高达50 mg/l。试验精密度为
We have developed a tandem mass spectrometry (LC-MS-MS) method for measuring tobramycin concentrations in serum samples and have compared it with a fluorescence polarisation immunoassay. After protein precipitation with acetonitrile supernatant was injected into the LC-MS-MS system. A C-18 cartridge (4 x 2 mm) was eluted with a step gradient of 20-100% methanol containing HFBA. The retention times were, tobramycin 1.05 min and sisomycin 1.05 min. The MRM transitions were: m/z 467.8 > 163 (tobramycin) and m/z 447.8 > 160 (sisomycin). The limit of quantification was 0.15 mg/l and the assay was linear up to 50 mg/l. Assay precision was