Nanoalumina promotes the horizontal transfer of multiresistance genes mediated by plasmids across genera
Nanoalumina promotes the horizontal transfer of multiresistance genes mediated by plasmids across genera
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DOI：
10.1073/pnas.1107254109
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发表时间：
2012-03-27
影响因子：
11.1
通讯作者：
Li, Jun-Wen
中科院分区：
文献类型：
--
作者：
Qiu, Zhigang;Yu, Yunmei;Li, Jun-Wen
抗生素耐药性是一个全球性的公共卫生问题。近缘菌株间的接合转移是多药耐药基因水平转移的重要途径。纳米材料能够通过调节细菌中抗生素抗性基因的接合转移（特别是跨属）来增加抗生素抗性的程度仍然未知。在这里，我们表明，在水中的纳米材料可以显着促进由RP 4，RK2和pCF 10质粒介导的多药耐药基因的水平接合转移。纳米氧化铝可以促进RP 4质粒从大肠杆菌到沙门氏菌的接合转移。与未经处理的细胞相比，增加了200倍。我们还探讨了这一现象背后的机制，并证明纳米氧化铝能够诱导氧化应激，破坏细菌细胞膜，增强交配对形成基因和DNA转移和复制基因的表达，并抑制调节RP4接合转移的全局调控基因的表达。这些发现对于评估纳米材料对环境的风险，特别是来自水和废水处理系统的风险，以及估计纳米材料的制造和使用对环境的影响都很重要。
Antibiotic resistance is a worldwide public health concern. Conjugative transfer between closely related strains or species of bacteria is an important method for the horizontal transfer of multidrug-resistance genes. The extent to which nanomaterials are able to cause an increase in antibiotic resistance by the regulation of the conjugative transfer of antibiotic-resistance genes in bacteria, especially across genera, is still unknown. Here we show that nanomaterials in water can significantly promote the horizontal conjugative transfer of multidrug-resistance genes mediated by the RP4, RK2, and pCF10 plasmids. Nanoalumina can promote the conjugative transfer of the RP4 plasmid from Escherichia coli to Salmonella spp. by up to 200-fold compared with untreated cells. We also explored the mechanisms behind this phenomenon and demonstrate that nanoalumina is able to induce oxidative stress, damage bacterial cell membranes, enhance the expression of mating pair formation genes and DNA transfer and replication genes, and depress the expression of global regulatory genes that regulate the conjugative transfer of RP4. These findings are important in assessing the risk of nanomaterials to the environment, particularly from water and wastewater treatment systems, and in the estimation of the effect of manufacture and use of nanomaterials on the environment.