Oxylipin analysis methods
Oxylipin analysis methods
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DOI：
10.1111/j.1365-313x.2005.02614.x
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发表时间：
2006-02-01
期刊：
影响因子：
7.2
通讯作者：
Farmer, EE
中科院分区：
文献类型：
--
作者：
Mueller, MJ;Mène-Saffrané, L;Farmer, EE
提出了监测和测量茉莉酸酯、酮醇、酮二(三)烯和羟基脂肪酸等化合物以及检测新型氧脂存在的实用指南。此外，还描述了一种用于非酶脂肪氧化渗透分析的方法。使用气相色谱/质谱仪的每一种方法都可以在没有专业知识或求助于最新分析仪器的情况下应用。提供了有关氧脂定量和制备氧同位素标记内标的新方案的更多信息。确定了四个正在开发的研究领域：(I)氧脂游离细胞池的分析；(Ii)酯化氧脂的分析和/或其从母体脂中的释放的监测；(Iii)非酶脂质氧化的监测；(Iv)不稳定和反应性氧脂的分析。本文提出的方法和协议旨在提供对前三个领域的技术见解，并提供进入第四个领域的平台。
Practical guidelines for monitoring and measuring compounds such as jasmonates, ketols, ketodi(tri)enes and hydroxy-fatty acids as well as detecting the presence of novel oxylipins are presented. Additionally, a protocol for the penetrant analysis of non-enzymatic lipid oxidation is described. Each of the methods, which employ gas chromatography/mass spectrometry, can be applied without specialist knowledge or recourse to the latest analytical instrumentation. Additional information on oxylipin quantification and novel protocols for preparing oxygen isotope-labelled internal standards are provided. Four developing areas of research are identified: (i) profiling of the unbound cellular pools of oxylipins; (ii) profiling of esterified oxylipins and/or monitoring of their release from parent lipids; (iii) monitoring of non-enzymatic lipid oxidation; (iv) analysis of unstable and reactive oxylipins. The methods and protocols presented herein are designed to give technical insights into the first three areas and to provide a platform from which to enter the fourth area.