Purification of an isoflavonoid 7-O-β-apiosyl-glucoside β-glycosidase and its substrates from Dalbergia nigrescens Kurz
Purification of an isoflavonoid 7-O-β-apiosyl-glucoside β-glycosidase and its substrates from Dalbergia nigrescens Kurz
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DOI：
10.1016/j.phytochem.2005.06.024
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发表时间：
2005-08-01
期刊：
影响因子：
3.8
通讯作者：
Cairns, JRK
中科院分区：
文献类型：
--
作者：
Chuankhayan, P;Hua, YL;Cairns, JRK
利用黄檀种子对对硝基苯基β-葡萄糖苷和β-岩藻糖苷的降解能力，从黄檀种子中分离纯化出一种β-糖苷酶。这种酶不能有效地降解各种糖苷底物，因此它被用来鉴定自己的天然底物。化合物1，‘-tetramethoxy-7-hydroxy-7-O-beta-(D)-apiofuranosyl-(1-(D)-葡萄糖苷和化合物2，6，2’，4‘，5-吡喃葡萄糖苷(dalnigrein7-O-β-(D)-iofuranosyl-(1-gt；6)-β-(D)-吡喃葡萄糖苷)。尽管β-葡萄糖苷酶最初被鉴定为β-葡萄糖苷酶和β-岩藻糖苷酶，但它还是以二糖的形式从这些糖苷中去除了糖。(C)2005爱思唯尔有限公司。保留所有权利。
A beta-glycosidase was purified from the seeds of Dalbergia nigescens Kurz based on its ability to hydrolyse p-nitrophenyl beta-glucoside and beta-fucoside. This enzyme did not hydrolyze various glycosidic substrates efficiently, so it was used to identify its own natural substrates. Two substrates were identified, isolated and their structures determined as: compound 1, dalpatein 7-0-p-D-apiofuranosyl-(l -> 6)-beta-(D)-glucopyranoside and compound 2, 6,2 ',4 ', 5 '-tetramethoxy-7-hydroxy-7-O-beta-(D)-apiofuranosyl-(1 -> 6)-beta-(D)-glucopyranoside (dalnigrein7-O-beta-(D)-iofuranosyl-(1 -> 6)-beta-(D)-glucopyranoside). The beta-glycosidase removes the sugar from these glycosides as a disaccharide, despite its initial identification as a beta-glucosidase and beta-fucosidase. (c) 2005 Elsevier Ltd. All rights reserved.