Precise characterization of GlnR Box in actinomycetes
Precise characterization of GlnR Box in actinomycetes
复制标题
放线菌中 GlnR Box 的精确表征
DOI：
10.1016/j.bbrc.2015.02.010
复制
发表时间：
2015-03-13
影响因子：
3.1
通讯作者：
Zhao, Guo-Ping
中科院分区：
文献类型：
--
作者：
Wang, Jin;Wang, Ying;Zhao, Guo-Ping
GlnR 被认为是许多重要放线菌中控制大多数氮代谢的中央调节因子。迄今为止，GlnR结合共有序列已被广泛研究，但提出了不同的基序，因此带来了混乱并阻碍了对GlnR介导的转录调控分子机制的深入理解。此处，采用地中海无枝酸菌 ginA 启动子中的 30 nt GlnR 保护的 DNA 序列来精确表征 GlnR 结合共有序列。采用逐位点诱变策略结合电泳迁移率变化分析，精确定义了5-nt GlnR Box作为GlnR结合的基本单位。 (C) 2015 Elsevier Inc. 保留所有权利。
GlnR has been characterized as a central regulator governing most nitrogen metabolisms in many important actinomycetes. So far, the GlnR binding consensus sequences have been extensively studied, but with different motifs proposed, which has therefore brought confusion and impeded the understanding of the in-depth molecular mechanisms of GlnR-mediated transcriptional regulation. Here, a 30-nt GlnR-protected DNA sequence in the promoter of ginA in Amycolatopsis mediterranei was employed for precise characterization of GlnR binding consensus sequences. Site-by-site mutagenesis strategy combining with the Electrophoretic Mobility Shift Assay were employed, and a 5-nt GlnR Box was precisely defined as the basic unit for GInR binding. (C) 2015 Elsevier Inc. All rights reserved.