Deep three-photon imaging of the brain in intact adult zebrafish
Deep three-photon imaging of the brain in intact adult zebrafish
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DOI：
10.1038/s41592-020-0819-7
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发表时间：
2020-04-27
期刊：
影响因子：
48
通讯作者：
Fetcho, Joseph R.
中科院分区：
文献类型：
--
作者：
Chow, Dawnis M.;Sinefeld, David;Fetcho, Joseph R.
三光子显微镜提供了进入大多数成年斑马鱼大脑的结构和功能成像，以及Danionella dracula brain.Behaviors出现从整个大脑的活动，但在成年脊椎动物大脑的非侵入性光学访问是有限的。我们表明，通过完整的成年斑马鱼头部的三光子（3P）成像允许在整个端脑和深入小脑和视顶盖的细胞分辨率的结构和功能成像。通过3P成像，在脊椎动物模型中，从胚胎到性成熟的成年人，大脑的相当大的部分变得可以无创地访问。
Three-photon microscopy provides access to most of the adult zebrafish brain for both structural and functional imaging, as well as to the Danionella dracula brain.Behaviors emerge from activity throughout the brain, but noninvasive optical access in adult vertebrate brains is limited. We show that three-photon (3P) imaging through the head of intact adult zebrafish allows structural and functional imaging at cellular resolution throughout the telencephalon and deep into the cerebellum and optic tectum. With 3P imaging, considerable portions of the brain become noninvasively accessible from embryo to sexually mature adult in a vertebrate model.