Green Preparation of S and N Co-Doped Carbon Dots from Water Chestnut and Onion as Well as Their Use as an Off On Fluorescent Probe for the Quantification and Imaging of Coenzyme A
Green Preparation of S and N Co-Doped Carbon Dots from Water Chestnut and Onion as Well as Their Use as an Off On Fluorescent Probe for the Quantification and Imaging of Coenzyme A
复制标题
从荸荠和洋葱中绿色制备 S 和 N 共掺杂碳点及其作为辅酶 A 定量和成像的关闭荧光探针的用途
DOI：
10.1021/acssuschemeng.7b00393
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发表时间：
2017-06-01
影响因子：
8.4
通讯作者：
Zhao, Shulin
中科院分区：
文献类型：
--
作者：
Hu, Yuefang;Zhang, Liangliang;Zhao, Shulin
来源于天然生物质的荧光碳点（CD）由于其上级的光学和化学性质，近年来引起了人们极大的兴趣。然而，以往报道的CD仅使用一种天然生物质作为前体，荧光量子产率（QY）和长波长发射通常较弱，这限制了其在生物学相关领域的进一步应用。以天然生物质（菱角和洋葱）为前驱体，采用绿色方法制备了硫氮共掺杂的荧光染料（S，N/CD）。制造过程简单且环保。通过水热加热菱角和洋葱，得到了单分散、高荧光的S，N/CD（直径3.5nm）。S，N/CD表面的羧基可以与Cu（II）离子结合，导致S，N/CD的发光猝灭。而辅酶A（CoA）可以恢复S，N/CD的发光。基于S，N/CD的上述特点，提出了一种新的高灵敏度测定辅酶A的关闭荧光探针。在最佳条件下，CoA检测的线性范围为0.03-40 μ M，检测限为0.01 μ M。将所开发的纳米探针应用于猪肝中CoA的定量和活T24细胞中CoA的成像。
Fluorescent carbon dots (CDs) originated from natural biomass have been of great interest in recent years because of their superior optical and chemical properties. However, previously reported CDs used only one natural biomass as precursor, and the fluorescence quantum yield (QY) and long -wavelength emissions are usually weak, which restrict their further applications in biology-relevant fields. Here a green method was demonstrated for the preparation of S and N codoped fluorescent CDs (S,N/CDs) by adopting two natural biomasses (water chestnut and onion) as precursors. The fabrication process is simple and environmentally friendly. By hydrothermal heating of water chestnut and onion, monodispersed, highly fluorescent S,N/CDs (diameter 3.5 nm) were obtained. The carboxyl on the surface of S,N/CDs can bind to Cu(II) ion, resulting in the luminescence quenching of S,N/CDs. And coenzyme A (CoA) can restore the luminescence of S,N/CDs. Based on the above features of S,N/CDs, an innovative off on fluorescence probe was presented for high sensitivity determination of CoA. Under optimum conditions, the linear range for CoA detection is 0.03-40 mu M with a detection limit of 0.01 mu M. The developed off on nanoprobe was applied for the quantification of CoA in pig liver, and imaging of CoA in living T24 cells.