Endothelial injury induces vascular smooth muscle cell proliferation in highly localized regions of a direct contact co-culture system.
Endothelial injury induces vascular smooth muscle cell proliferation in highly localized regions of a direct contact co-culture system.
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DOI：
10.1007/s12013-008-9023-6
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发表时间：
2008
影响因子：
2.6
通讯作者：
Wong, Joyce Y.
中科院分区：
文献类型：
--
作者：
Jacot, Jeffrey G.;Wong, Joyce Y.
关键词：
虽然先前的研究表明，在共培养中内皮细胞和血管平滑肌细胞(VSMCs)的增殖之间存在关系，但结果是相互矛盾的，信号机制也鲜为人知。在这项跨膜共培养研究中，血管平滑肌细胞和内皮细胞在微孔膜的两侧融合生长，以模拟血管的内膜/中膜边界。损伤内皮层，培养3天，部分再内皮化。损伤/部分恢复内皮区的VSMC增殖明显高于去内皮化区(增加7倍)和未损伤区(增加3倍)。ELISA法显示，在未损伤的共培养细胞和同型对照中未检测到的PDGF在损伤后增加，加入哌嗪基喹唑啉羧胺PDGF受体抑制剂可阻断损伤/部分恢复区域的VSMC增殖。结论：内皮细胞损伤引起的共培养信号局部刺激VSMC增殖，此信号转导可能由PDGF-BB介导。
Though previous studies have indicated a relationship between the proliferation of endothelial cells and vascular smooth muscle cells (VSMCs) in co-culture, the results have been contradictory and the signaling mechanism poorly understood. In this transmembrane co-culture study, VSMCs and endothelial cells were grown to confluence on opposite sides of a microporous membrane to mimic the intima/media border of vessels. The endothelial layer was injured, and then cultured for three days, resulting in partial re-endothelialization. VSMC proliferation across from the injured/partially recovered endothelial region was significantly higher than across from the de-endothelialized region (a 7-fold increase) and the uninjured region (a 3-fold increase). ELISA indicated that PDGF, which was undetectable in uninjured co-culture and homotypic controls, increased after injury and the addition of a piperazinylquinazoline carboxamide PDGF receptor inhibitor blocked VSMC proliferation across from the injured/partially recovered region. We conclude that co-culture signaling initiated by endothelial cell injury locally stimulates VSMC proliferation and that this signaling could be mediated by PDGF-BB.
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DOI：
10.1073/pnas.0408919102
发表时间：
2005-05-10
影响因子：
11.1
作者：
Griffith, TM;Chaytor, AT;Edwards, DH
通讯作者：
Edwards, DH
影响因子：
8.7
作者：
Chen, DH;Walsh, K;Wang, J
通讯作者：
Wang, J
影响因子：
4.3
作者：
Dardik, A;Yamashita, A;Sumpio, BE
通讯作者：
Sumpio, BE
影响因子：
2.2
作者：
Cucina, A;Borrelli, V;Cavallaro, A
通讯作者：
Cavallaro, A
DOI：
10.1111/j.1749-6632.1995.tb26691.x
发表时间：
1995-01-01
期刊：
RECEPTOR ACTIVATION BY ANTIGENS, CYTOKINES, HORMONES, AND GROWTH FACTORS
影响因子：
--
作者：
Bornfeldt, KE;Raines, EW;Ross, R
通讯作者：
Ross, R