Large-scale oligoribonucleotide production
Large-scale oligoribonucleotide production
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DOI：
10.1016/0958-1669(95)80035-2
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发表时间：
1995-01-01
影响因子：
7.7
通讯作者：
Davis, Robert H.
中科院分区：
文献类型：
--
作者：
Davis, Robert H.
对用于生物医学、农业和其他应用的RNA的日益增长的兴趣使得开发用于以毫克或更大的量生产寡核糖核苷酸的方法成为必要。体外转录的最新研究集中在优化溶液条件和使用固定化模板。在有机化学合成的替代领域中，已经将显著的努力引导到改善偶联效率和产生修饰的RNA类似物，其保留活性但具有比未修饰的RNA更高的稳定性。
Growing interest in RNAs for biomedical, agricultural, and other applications has necessitated the development of methods for producing oligoribonucleotides in milligram, or larger, quantities. Recent research on in vitro transcription has focused on optimizing solution conditions and the use of immobilized templates. In the alternative area of organic chemical synthesis, significant effort has been channeled into improving coupling efficiencies and producing modified RNA analogs which retain activity but have higher stability than unmodified RNAs.