Early response of neural stem/progenitor cells after X-ray irradiation in vitro
Early response of neural stem/progenitor cells after X-ray irradiation in vitro
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DOI：
10.1097/wnr.0b013e3281053c34
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发表时间：
2007-06-11
期刊：
影响因子：
1.7
通讯作者：
Hara, Masayuki
中科院分区：
文献类型：
--
作者：
Kato, Tomoaki;Kanemura, Yonehiro;Hara, Masayuki
我们研究了氧化应激对神经球培养的神经干/祖细胞的细胞周期调控的影响。我们将小鼠神经干/祖细胞在第一次传代后48小时暴露于2戈伊的X射线照射。激光扫描细胞仪检测发现，X射线照射后3和12 h，G2期和GI期阻滞细胞分别增加。我们发现，这种G2和G1期阻滞与cdc 2和p53的磷酸化，分别通过Western印迹分析。此外，我们发现X射线照射神经干/祖细胞的影响涉及Notch信号的失活。这些结果表明，神经干/祖细胞在X射线照射后，即使在短时间内也会发生剧烈的反应。
We investigated the effect of oxidative stress on cell cycle regulation of neural stem/progenitor cells in neurosphere culture. We exposed murine neural stem/progenitor cells to 2 Gy of X-ray irradiation at 48 h after first passage. We found that G2 and GI-arrested cells increased at 3 and 12h after X-ray irradiation, respectively by using laser scanning cytometer. We revealed that such G2 and G1 arrests were correlated with phosphorylation of cdc2 and p53, respectively by Western blotting analysis. Furthermore, we found that the effects of X-ray irradiation of neural stem/progenitor cells involved inactivation of Notch signal. These results suggest that the drastic response of neural stem/ progenitor cells after X-ray irradiation occurred even in the short period.