TURGOR-RESPONSIVE GENE-TRANSCRIPTION AND RNA LEVELS INCREASE RAPIDLY WHEN PEA SHOOTS ARE WILTED - SEQUENCE AND EXPRESSION OF 3-INDUCIBLE GENES
TURGOR-RESPONSIVE GENE-TRANSCRIPTION AND RNA LEVELS INCREASE RAPIDLY WHEN PEA SHOOTS ARE WILTED - SEQUENCE AND EXPRESSION OF 3-INDUCIBLE GENES
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DOI：
10.1007/bf00017720
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发表时间：
1990-07-01
影响因子：
5.1
通讯作者：
MULLET, JE
中科院分区：
文献类型：
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作者：
GUERRERO, FD;JONES, JT;MULLET, JE
豌豆芽膨压的减少引起了几种poly（A）RNA的积累。从萎蔫豌豆苗中积累的三种不同的poly（A）RNA中分离cDNA克隆，测序并检测相应基因的表达。克隆7a编码289个氨基酸的蛋白。该蛋白的C末端180个氨基酸与大豆结瘤素-26同源。与cDNA 7a杂交的RNA在根中丰富，在芽中受脱水、热激和少量阿坝诱导。亲水性图表明，cDNA 7a编码的蛋白质含有六个潜在的跨膜结构域的蛋白质，形成离子通道。克隆15 a编码一个363个氨基酸的蛋白，与半胱氨酸蛋白酶具有高度同源性。与cDNA 15 a杂交的RNA在对照植物的根中比在地上部中更丰富。脱水豌豆芽诱导cDNA 15 a mRNA水平，而热休克或阿坝处理没有。克隆26 g编码508个氨基酸的蛋白质，与几种乙醛脱氢酶的同源性为30%。与cDNA 26 g杂交的RNA由芽的脱水而不是根诱导，热激和阿坝不调节RNA水平。萎蔫后4小时，三种poly（A）RNA的水平增加了4-6倍，这种增加并没有改变用放线菌酮预处理的芽。当萎蔫的芽再水化时，与cDNA 26 g结合的RNA在2 h内下降到胁迫前的水平。使用豌豆芽细胞核的连续转录实验表明，编码三种poly（A）RNA的基因的转录在芽膨压降低后30分钟内被诱导。其中一个基因在脱水后4 h转录进一步增加，而其他2个基因的转录下降。这些结果表明，植物细胞通过快速增加几个基因的转录来响应细胞膨压的变化。此外，turgor-responsive基因的表达随着诱导的时间过程和萎蔫诱导的变化的可逆性而变化。
Reduction of turgor in pea shoots caused the accumulation of several poly(A) RNAs. cDNA clones derived from three different poly(A) RNAs which accumulated in wilted pea shoots were isolated, sequenced and expression of the corresponding genes examined. Clone 7a encoded a 289 amino acid protein. The C-terminal 180 amino acids of this protein were homologous to soybean nodulin-26. RNA hybridizing to cDNA 7a was abundant in roots, and induced in shoots by dehydration, heat shock and to a small extent by ABA. Hydropathic plots indicate that the protein encoded by cDNA 7a contains six potential membrane spanning domains to proteins which form ion channels. Clone 15a encoded a 363 amino acid protein with high homology to cysteine proteases. RNA hybridizing to cDNA 15a was more abundant in roots than shoots of control plants. Dehydration of pea shoots induced cDNA 15a mRNA levels whereas heat shock or ABA treatment did not. Clone 26g encoded a 508 amino acid protein with 30% residue identity to several aldehyde dehydrogenases. RNA hybridizing to cDNA 26g was induced by dehydration of shoots but not roots and heat shock and ABA did not modulate RNA levels. Levels of the three poly(A) RNAs increased 4-6-fold by 4 h after wilting and this increase was not altered by pretreatment of shoots with cycloheximide. When wilted shoots were rehydrated, RNA hydridizing to cDNA 26g declined to pre-stress levels within 2 h. Run-on transcription experiments using nuclei from pea shoots showed that transcription of the genes which encode the three poly (A)RNAs was induced within 30 min following reduction of shoot turgor. One of the genes showed a further increase in transcription by 4 h after dehydration whereas transcription of the other 2 genes declined. These results indicate that plants cells respond to changes in cell turgor by rapidly increasing trancription of several genes. Furthermore, the expression of the turgor-responsive genes varies with respect to the time course of induction and reversibility of the wilting-induced changes.