Improved High-Force Magnetic Tweezers for Stretching and Refolding of Proteins and Short DNA
Improved High-Force Magnetic Tweezers for Stretching and Refolding of Proteins and Short DNA
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DOI：
10.1016/j.bpj.2010.12.3700
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发表时间：
2011-01-19
影响因子：
3.4
通讯作者：
Yan, Jie
中科院分区：
文献类型：
--
作者：
Chen, Hu;Fu, Hongxia;Yan, Jie
Although magnetic tweezers have many unique advantages in terms of specificity, throughput, and force stability, this tool has had limited application on short tethers because accurate measurement of force has been difficult for short tethers under large tension. Here, we report a method that allows us to apply magnetic tweezers to stretch short biomolecules with accurate force calibration over a wide range of up to 100 pN. We demonstrate the use of the method by overstretching of a short DNA and unfolding/refolding a protein of filamin A immunoglobulin domains 1-8. Other potential applications of this method are also discussed.
