Rapid Fiber-optic Raman Spectroscopy for Real-Time In Vivo Detection of Gastric Intestinal Metaplasia during Clinical Gastroscopy
Rapid Fiber-optic Raman Spectroscopy for Real-Time In Vivo Detection of Gastric Intestinal Metaplasia during Clinical Gastroscopy
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DOI：
10.1158/1940-6207.capr-15-0213
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发表时间：
2016-06-01
影响因子：
3.3
通讯作者：
Huang, Zhiwei
中科院分区：
文献类型：
--
作者：
Lin, Kan;Wang, Jianfeng;Huang, Zhiwei
我们报告了一种独特的同时指纹（FP）和高波数（HW）拉曼光谱技术，结合斜光纤拉曼探针，用于改善临床胃镜检查过程中实时检测胃肠上皮化生（IM）-癌前病变。从157名接受内窥镜检查的胃患者中获得了总计4，520个高质量的体内FP/HW胃拉曼光谱（正常= 4，178; IM = 342）。基于主成分分析和线性判别分析的多元诊断算法，结合留一法和交叉验证法，对活体组织拉曼光谱进行诊断，其诊断灵敏度分别为89.3%、89.3%和75.0%，特异度分别为92.2%、84.4%和82.0%;通过使用FP/HW、FP和HW拉曼综合技术从正常胃组织中鉴别IM，阳性预测值分别为52.1%、35.2%和28.4%，阴性预测值分别为98.9%、98.8%和97.2%。此外，生成的ROC曲线显示，对于IM分类，集成FP/HW拉曼技术给出0.92的ROC曲线下的积分面积，这上级单独的FP（0.89）或HW拉曼（0.86）技术。这项工作表明，第一次，同时FP/HW光纤拉曼光谱有很大的潜力，以提高早期诊断胃癌前病变在体内常规内镜检查。(C)2016年AACR。
We report a unique simultaneous fingerprint (FP) and high-wavenumber (HW) Raman spectroscopy technique coupled with a beveled fiber-optic Raman probe for improving in vivo detection of gastric intestinal metaplasia (IM)-precancerous lesions in real-time during clinical gastroscopy. A total of 4,520 high-quality in vivo FP/HW gastric Raman spectra (normal = 4,178; IM = 342) were acquired from 157 gastric patients undergoing endoscopic examination. Multivariate diagnostic algorithms based on principal components analysis and linear discriminant analysis together with the leave-one tissue site-out, cross-validation on in vivo tissue Raman spectra yield the diagnostic sensitivities of 89.3%, 89.3%, and 75.0%; specificities of 92.2%, 84.4%, and 82.0%; positive predictive values of 52.1%, 35.2%, and 28.4%; and negative predictive values of 98.9%, 98.8%, and 97.2%, respectively, by using the integrated FP/HW, FP, and HW Raman techniques for identifying IM from normal gastric tissue. Further, ROC curves generated show that the integrated FP/HW Raman technique gives the integration area under the ROC curve of 0.92 for IM classification, which is superior to either FP (0.89) or HW Raman (0.86) technique alone. This work demonstrates for the first time that the simultaneous FP/HW fiber-optic Raman spectroscopy has great potential to enhance early diagnosis of gastric precancer in vivo during routine endoscopic examination. (C) 2016 AACR.