Clinicopathological features and prognostic implications of Raf kinase inhibitor protein downregulation in tongue squamous cell carcinoma.
Clinicopathological features and prognostic implications of Raf kinase inhibitor protein downregulation in tongue squamous cell carcinoma.
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DOI：
10.3892/ol.2015.3496
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发表时间：
2015-09
期刊：
影响因子：
2.9
通讯作者：
F. Hu;Xiaohua Chen;Xiqiang Liu;Cheng Wang;Lanhai Lv;N. Xie;Tianyi Wang;H. Huang
中科院分区：
文献类型：
--
作者：
F. Hu;Xiaohua Chen;Xiqiang Liu;Cheng Wang;Lanhai Lv;N. Xie;Tianyi Wang;H. Huang
RAF激酶抑制蛋白(RKIP)被认为是一种转移抑制因子，在多种实体肿瘤中，RKIP表达下调与侵袭性事件和不良预后有关。然而，RKIP在舌鳞状细胞癌(TSCC)中的表达与临床相关性尚不清楚。本研究采用免疫组织化学方法检测了85对TSCC及相应癌旁组织、30例匹配的颈淋巴转移灶和32例口腔白斑标本中RKIP的表达。然后评估RKIP表达与临床病理特征之间的关系。采用Kaplan-Meier生存分析和Cox比例风险模型分析RKIP表达对TSCC患者生存时间的影响。结果表明，RKIP在TSCC中的表达显著下调，在转移灶中的下调程度更大。RKIP下调与TSCC的淋巴转移和临床分期显著相关。此外，RKIP低表达的患者总体生存时间显著缩短。多因素分析显示，RKIP表达可能是影响TSCC预后的独立因素。综上所述，目前的研究结果表明，RKIP的表达缺失具有临床意义，可作为预测TSCC预后的生物标志物。
Raf kinase inhibitor protein (RKIP) is recognized as a suppressor of metastasis, and the downregulation of RKIP is associated with aggressive events and a poor outcome in a variety of solid tumors. However, the clinical relevance of RKIP expression in tongue squamous cell carcinoma (TSCC) remains unclear. In the present study, the expression of RKIP in 85 pairs of TSCC and corresponding adjacent non-cancerous tissues, 30 matched metastatic lesions from the cervical lymph nodes and 32 oral leukoplakia samples were assessed using immunohistochemical methods. The association between RKIP expression and clinicopathological features was then evaluated. Kaplan-Meier survival analysis and Cox proportional hazards model were used to estimate the effect of RKIP expression on the survival time of patients with TSCC. The results revealed that RKIP expression was dramatically downregulated in TSCC, and to an even greater extent in metastatic lesions. RKIP downregulation was significantly associated with the presence of lymphatic metastasis and the clinical stage of TSCC. Furthermore, patients with low RKIP expression demonstrated a significantly shorter overall survival time. Multivariate analysis indicated that RKIP expression may be an independent prognostic factor in TSCC. In conclusion, the present findings indicate that the lack of RKIP expression is of clinical significance and may serve as a prognostic biomarker in TSCC.