Analysis of Genetic Polymorphism of Deoxyribonuclease I in Ovambo and Turk Populations Using a Genotyping Method
Analysis of Genetic Polymorphism of Deoxyribonuclease I in Ovambo and Turk Populations Using a Genotyping Method
复制标题
采用基因分型方法分析 Ovambo 和 Turk 人群脱氧核糖核酸酶 I 的遗传多态性
作者：
J. Fujihara;Y. Hieda;K. Takayama;Y. Xue;N. Nakagami;S. Imamura;K. Kataoka;H. Takeshita
脱氧核糖核酸酶I（DNase I）多态性已被用作遗传和临床研究中有价值的标记。已知六个共显性等位基因为DNA酶I、DNA酶1、DNA酶2、DNA酶3、DNA酶4以及最近发现的等位基因DNA酶5和DNA酶6。为了检测这两个新的等位基因，我们增加了一个新的DNA酶I基因分型方法的基础上，等位基因特异性扩增和错配聚合酶链反应（PCR）。这些方法被用来检查分布的DNA酶I基因型无关的个体从血迹的Ovambo和土耳其人口。DNASE1等位基因在Ovambos中占优势。相比之下，土耳其人显示出最高的等位基因频率为DNASE1 β 2。本研究首次用PCR方法对人类DNA酶I多态性进行基因分型，证明DNA酶I多态性在世界范围内的分布存在一定的遗传异质性。
Deoxyribonuclease I (DNase I) polymorphism has been used as a valuable marker in genetic and clinical investigations. Six codominant alleles are known for DNase I, DNASE1∗1, ∗2, ∗3, ∗4, and the recently discovered alleles ∗5 and ∗6. To detect these two new alleles, we added a new DNase I genotyping method based on both an allele-specific amplification and mismatched polymerase chain reaction (PCR). These methods were used to examine the distribution of DNase I genotypes in unrelated individuals from bloodstains of Ovambo and Turkish populations. The DNASE1∗1 allele was found to be most dominant in the Ovambos. In contrast, Turks showed the highest allele frequency for DNASE1∗2. This study is the first to demonstrate that there is a certain genetic heterogeneity in the worldwide distribution of DNase I polymorphism using the genotyping method of human DNase I polymorphism with PCR.
设计了诊断性限制性片段长度多态性，用于检测 ras 癌基因中的点突变。
DOI：
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发表时间：
1989
期刊：
Oncogene research
影响因子：
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作者：
Kumar,R;Dunn,LL
通讯作者：
Dunn,LL